Wang Guilan, Gu Xiaolin, Chen Li, Wang Yinmei, Cao Bin, E Qun
Department of Pathological Anatomy, Medical College, Nantong University, Nantong, P.R. China.
Oncol Lett. 2013 Apr;5(4):1363-1369. doi: 10.3892/ol.2013.1166. Epub 2013 Jan 30.
The objective of this study was to analyze the significance and potential value of heat shock proteins (HSPs) in salivary gland tumors. We found that expression of HSP60, HSP70, HSP86 and HSP84 were all upregulated in both salivary gland benign tumors and malignant tumors, and that the expression of HSP70, HSP86 and HSP84 was more greatly overexpressed in the malignant tumors (each P<0.01). For HSP27, expression was upregulated both in malignant and benign tumors, with less expression observed in malignant tumors (P<0.01). In malignant tumors, expression of HSP27 was negatively correlated with the age of the patients, size of the tumor tissue, occurrence of neural invasion and metastasis (each P<0.05). Additionally, in malignant tumors, HSP70 and HSP86 were both positively correlated with occurrence site, neural invasion and metastasis (each P<0.05), while HSP60 was only negatively correlated with the age of the patients (P<0.05). HSP86 was also positively correlated with malignant degree (P<0.01). In malignant tumors, the proliferation index (PI), which was marked by proliferating cell nuclear antigen (PCNA; PCNA-PI) was 49.95±14.569, which was significantly higher compared with that in benign tumors (P<0.001), which was in accordance with the upregulation of HSP70, HSP86 or HSP84; however, an adverse correlation was found between HSP27 expression and PCNA (each P<0.05). In conclusion, these results suggest that HSPs are involved in the occurrence and development of salivary gland tumors. HSP70, HSP86 and HSP84 retained the higher multiplication capability of the malignant tumor cells, however, HSP27 did not. Thus, the upregulation of HSP70, HSP86 and HSP84 and the downregulation of HSP27 may all be used as biomarkers of the occurrence and development of malignant salivary gland tumors. Moreover, the extremely high expression of HSP86 and HSP84 in benign tumors indicates the malignant transformation potential.
本研究的目的是分析热休克蛋白（HSPs）在涎腺肿瘤中的意义和潜在价值。我们发现，HSP60、HSP70、HSP86和HSP84在涎腺良性肿瘤和恶性肿瘤中均上调，且HSP70、HSP86和HSP84在恶性肿瘤中的表达上调更为明显（各P<0.01）。对于HSP27，其在恶性和良性肿瘤中均上调，但在恶性肿瘤中的表达较少（P<0.01）。在恶性肿瘤中，HSP27的表达与患者年龄、肿瘤组织大小、神经侵犯和转移的发生均呈负相关（各P<0.05）。此外，在恶性肿瘤中，HSP70和HSP86均与发生部位、神经侵犯和转移呈正相关（各P<0.05），而HSP60仅与患者年龄呈负相关（P<0.05）。HSP86也与恶性程度呈正相关（P<0.01）。在恶性肿瘤中，以增殖细胞核抗原（PCNA；PCNA-PI）标记的增殖指数（PI）为49.95±14.569，与良性肿瘤相比显著更高（P<0.001），这与HSP70、HSP86或HSP84的上调一致；然而，发现HSP27表达与PCNA之间存在负相关（各P<0.05）。总之，这些结果表明HSPs参与了涎腺肿瘤的发生和发展。HSP70、HSP86和HSP84保留了恶性肿瘤细胞较高的增殖能力，然而，HSP27并非如此。因此，HSP70、HSP86和HSP84的上调以及HSP27的下调均可用作恶性涎腺肿瘤发生和发展的生物标志物。此外，HSP86和HSP84在良性肿瘤中的极高表达表明其具有恶性转化潜能。
