在靶标活细胞表面构建荧光 DNA 纳米器件。
Building fluorescent DNA nanodevices on target living cell surfaces.
机构信息
Molecular Science and Biomedicine Laboratory, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, 410082, China.
出版信息
Angew Chem Int Ed Engl. 2013 May 17;52(21):5490-6. doi: 10.1002/anie.201301439. Epub 2013 Apr 18.
Abstract
We report 1) the anchoring of preformed fluorescent DNA nanodevices (NDs) and 2) the self-assembly of fluorescent DNA NDs on target living cell surfaces. Three types of aptamer-tethered DNA NDs were built and anchored on target cell surfaces by specific target-aptamer association. The nanodevice self-assembly was further demonstrated on the surfaces of target living cells in cell mixtures. These DNA NDs exhibited fluorescence emission and underwent fluorescence energy transfer on living cell surfaces.
摘要
我们报告了 1）预先形成的荧光 DNA 纳米器件 (NDs) 的固定，以及 2）荧光 DNA NDs 在靶活细胞表面的自组装。构建了三种适体连接的 DNA NDs，并通过特定的靶适体结合固定在靶细胞表面。进一步在细胞混合物中靶活细胞表面上证明了纳米器件的自组装。这些 DNA NDs 在活细胞表面上表现出荧光发射，并发生荧光能量转移。
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