Ryerson University, Department of Electrical and Computer Engineering, 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada.
J Biomed Opt. 2013 May;18(5):50901. doi: 10.1117/1.JBO.18.5.050901.
High-resolution mapping of microvasculature has been applied to diverse body systems, including the retinal and choroidal vasculature, cardiac vasculature, the central nervous system, and various tumor models. Many imaging techniques have been developed to address specific research questions, and each has its own merits and drawbacks. Understanding, optimization, and proper implementation of these imaging techniques can significantly improve the data obtained along the spectrum of unique research projects to obtain diagnostic clinical information. We describe the recently developed algorithms and applications of two general classes of microvascular imaging techniques: speckle-variance and phase-variance optical coherence tomography (OCT). We compare and contrast their performance with Doppler OCT and optical microangiography. In addition, we highlight ongoing work in the development of variance-based techniques to further refine the characterization of microvascular networks.
微血管高分辨率成像已应用于多种身体系统，包括视网膜和脉络膜血管、心脏血管、中枢神经系统和各种肿瘤模型。已经开发出许多成像技术来解决特定的研究问题，每种技术都有其自身的优点和缺点。了解、优化和正确实施这些成像技术可以显著改善在独特研究项目范围内获得的诊断临床信息。我们描述了两种微血管成像技术（散斑方差和相位方差光相干断层扫描 (OCT)）的最新算法和应用。我们比较和对比了它们与多普勒 OCT 和光学微血管造影的性能。此外，我们还强调了基于方差的技术的开发工作，以进一步细化微血管网络的特征描述。
