Department of Veterinary Clinical and Animal Sciences, University of Copenhagen, Groennegaardsvej 3, Frederiksberg, Copenhagen, 1870, Denmark.
Nanoscale Res Lett. 2013 Apr 26;8(1):195. doi: 10.1186/1556-276X-8-195.
Angiogenesis is vital for tumour formation, development and metastasis. Recent reports show that carbon nanomaterials inhibit various angiogenic signalling pathways and, therefore, can be potentially used in anti-angiogenic therapy. In the present study, we compared the effect of different carbon nanomaterials on blood vessel development. Diamond nanoparticles, graphite nanoparticles, graphene nanosheets, multi-wall nanotubes and C60 fullerenes were evaluated for their angiogenic activities using the in ovo chick embryo chorioallantoic membrane model. Diamond nanoparticles and multi-wall nanotubes showed the greatest anti-angiogenic properties. Interestingly, fullerene exhibited the opposite effect, increasing blood vessel development, while graphite nanoparticles and graphene had no effect. Subsequently, protein levels of pro-angiogenic growth factor receptors were analysed, showing that diamond nanoparticles decreased the expression of vascular endothelial growth factor receptor. These results provide new insights into the biological activity of carbon nanomaterials and emphasise the potential use of multi-wall nanotubes and diamond nanoparticles in anti-angiogenic tumour therapy.
血管生成对于肿瘤的形成、发展和转移至关重要。最近的报告表明，碳纳米材料抑制多种血管生成信号通路，因此可潜在用于抗血管生成治疗。在本研究中，我们比较了不同碳纳米材料对血管发育的影响。使用鸡胚绒毛尿囊膜模型评估了金刚石纳米颗粒、石墨纳米颗粒、石墨烯纳米片、多壁碳纳米管和 C60 富勒烯的血管生成活性。金刚石纳米颗粒和多壁碳纳米管表现出最强的抗血管生成特性。有趣的是，富勒烯表现出相反的效果，增加了血管发育，而石墨纳米颗粒和石墨烯则没有影响。随后，分析了促血管生成生长因子受体的蛋白水平，结果表明金刚石纳米颗粒降低了血管内皮生长因子受体的表达。这些结果为碳纳米材料的生物学活性提供了新的见解，并强调了多壁碳纳米管和金刚石纳米颗粒在抗血管生成肿瘤治疗中的潜在用途。
