从小麦草中分离出的具有免疫活性的低聚糖通过 Toll 样受体 2 信号调节单核细胞。
The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling.
机构信息
Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei 115, Taiwan.
出版信息
J Biol Chem. 2013 Jun 14;288(24):17689-97. doi: 10.1074/jbc.M112.448381. Epub 2013 Apr 29.
Abstract
Wheatgrass is one of the most widely used health foods, but its functional components and mechanisms remain unexplored. Herein, wheatgrass-derived oligosaccharides (WG-PS3) were isolated and found to induce CD69 and Th1 cytokine expression in human peripheral blood mononuclear cells. In particular, WG-PS3 directly activated the purified monocytes by inducing the expression of CD69, CD80, CD86, IL-12, and TNF-α but affected NK and T cells only in the presence of monocytes. After further purification and structural analysis, maltoheptaose was identified from WG-PS3 as an immunomodulator. Maltoheptaose activated monocytes via Toll-like receptor 2 (TLR-2) signaling, as discovered by pretreatment of blocking antibodies against Toll-like receptors (TLRs) and also determined by click chemistry. This study is the first to reveal the immunostimulatory component of wheatgrass with well defined molecular structures and mechanisms.
摘要
麦苗是应用最广泛的保健食品之一，但它的功能成分和作用机制仍不清楚。在此，我们分离出了麦苗来源的低聚糖（WG-PS3），并发现其能诱导人外周血单个核细胞表达 CD69 和 Th1 细胞因子。特别是，WG-PS3 通过诱导 CD69、CD80、CD86、IL-12 和 TNF-α 的表达直接激活纯化的单核细胞，但仅在存在单核细胞的情况下才影响 NK 和 T 细胞。经过进一步的纯化和结构分析，从 WG-PS3 中鉴定出麦芽七糖是一种免疫调节剂。麦芽七糖通过 Toll 样受体 2（TLR-2）信号通路激活单核细胞，这是通过预处理针对 Toll 样受体（TLRs）的阻断抗体和点击化学实验确定的。这项研究首次揭示了具有明确分子结构和作用机制的麦苗的免疫刺激成分。
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