连续波激光激发的受激拉曼散射成像。
Stimulated Raman scattering imaging by continuous-wave laser excitation.
机构信息
Hefei National Laboratory for Physical Sciences at Microscale and School of Life Science, University of Science and Technology of Science, Hefei 230027, China.
出版信息
Opt Lett. 2013 May 1;38(9):1479-81. doi: 10.1364/OL.38.001479.
Abstract
We demonstrate a low-cost-stimulated Raman scattering (SRS) microscope using continuous-wave (cw) lasers as excitation sources. A dual modulation scheme is used to remove the electronic background. The cw-SRS imaging of lipids in fatty liver is demonstrated by excitation of C─H stretch vibration.
摘要
我们展示了一种使用连续波 (cw) 激光作为激发源的低成本受激拉曼散射 (SRS) 显微镜。采用双调制方案去除电子背景。通过激发 C─H 伸缩振动，实现了脂肪肝中脂质的 cw-SRS 成像。
相似文献
1
Stimulated Raman scattering imaging by continuous-wave laser excitation.连续波激光激发的受激拉曼散射成像。
Opt Lett. 2013 May 1;38(9):1479-81. doi: 10.1364/OL.38.001479.
2
Time-lens based hyperspectral stimulated Raman scattering imaging and quantitative spectral analysis.基于时间透镜的高光谱受激拉曼散射成像与定量光谱分析。
J Biophotonics. 2013 Oct;6(10):815-20. doi: 10.1002/jbio.201300005. Epub 2013 Jul 10.
3
Comparison of continuous wave versus picosecond SRS and the resonance SRS effect.连续波与皮秒 SRS 及共振 SRS 效应的比较。
Appl Opt. 2020 Jan 20;59(3):622-627. doi: 10.1364/AO.59.000622.
4
Stimulated Raman photoacoustic imaging.受激拉曼光声成像。
Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20335-9. doi: 10.1073/pnas.1012432107. Epub 2010 Nov 8.
5
Fast denoising and lossless spectrum extraction in stimulated Raman scattering microscopy.在受激拉曼散射显微镜中快速降噪和无损光谱提取。
J Biophotonics. 2021 Aug;14(8):e202100080. doi: 10.1002/jbio.202100080. Epub 2021 May 24.
6
Shot-Noise-Limited Two-Color Stimulated Raman Scattering Microscopy with a Balanced Detection Scheme.采用平衡检测方案的散粒噪声限制双色受激拉曼散射显微镜
J Phys Chem B. 2020 Apr 2;124(13):2591-2599. doi: 10.1021/acs.jpcb.0c01065. Epub 2020 Mar 24.
7
Clinical Translation of Stimulated Raman Histology.受激拉曼组织学的临床转化。
Methods Mol Biol. 2022;2393:225-236. doi: 10.1007/978-1-0716-1803-5_12.
8
Compact, low-noise, all-solid-state laser system for stimulated Raman scattering microscopy.用于受激拉曼散射显微镜的紧凑型、低噪声全固态激光系统。
Opt Lett. 2015 Feb 15;40(4):593-6. doi: 10.1364/OL.40.000593.
9
Plasmon-enhanced stimulated Raman scattering microscopy with single-molecule detection sensitivity.等离子体增强受激拉曼散射显微镜具有单分子检测灵敏度。
Nat Commun. 2019 Nov 21;10(1):5318. doi: 10.1038/s41467-019-13230-1.
10
Ex-CARS: exotic configuration for coherent anti-Stokes Raman scattering microspectroscopy utilizing two laser sources.Ex-CARS：利用两个激光源的相干反斯托克斯拉曼散射微光谱学的外差配置。
J Biophotonics. 2010 Oct;3(10-11):653-9. doi: 10.1002/jbio.201000042.
引用本文的文献
1
Flickering nanometre-scale disorder in a crystal lattice tracked by plasmonic flare light emission.通过等离子体耀斑光发射追踪晶格中闪烁的纳米级无序现象。
Nat Commun. 2020 Feb 3;11(1):682. doi: 10.1038/s41467-019-14150-w.
2
Continuous-Wave Coherent Raman Spectroscopy via Plasmonic Enhancement.基于等离子体增强的连续波相干拉曼光谱学
Sci Rep. 2019 Aug 20;9(1):12092. doi: 10.1038/s41598-019-48573-8.
3
Label-Free Biomedical Imaging Using High-Speed Lock-In Pixel Sensor for Stimulated Raman Scattering.基于高速锁相像素传感器的无标记生物医学成像用于受激拉曼散射。
Sensors (Basel). 2017 Nov 9;17(11):2581. doi: 10.3390/s17112581.
4
Nrg4 promotes fuel oxidation and a healthy adipokine profile to ameliorate diet-induced metabolic disorders.Nrg4 可促进燃料氧化和健康的脂肪细胞因子谱，改善饮食诱导的代谢紊乱。
Mol Metab. 2017 Jun 21;6(8):863-872. doi: 10.1016/j.molmet.2017.03.016. eCollection 2017 Aug.
5
Development of a classification model for non-alcoholic steatohepatitis (NASH) using confocal Raman micro-spectroscopy.使用共聚焦拉曼显微光谱法开发非酒精性脂肪性肝炎（NASH）的分类模型。
J Biophotonics. 2017 Dec;10(12):1703-1713. doi: 10.1002/jbio.201600303. Epub 2017 Jun 21.
6
Pure electrical, highly-efficient and sidelobe free coherent Raman spectroscopy using acousto-optics tunable filter (AOTF).采用声光可调滤光器（AOTF）的纯电学、高效且无旁瓣的相干拉曼光谱技术。
Sci Rep. 2016 Feb 1;6:20017. doi: 10.1038/srep20017.
7
Rapid spontaneous Raman light sheet microscopy using cw-lasers and tunable filters.使用连续波激光器和可调谐滤光片的快速自发拉曼光片显微镜术。
Biomed Opt Express. 2015 Aug 19;6(9):3449-61. doi: 10.1364/BOE.6.003449. eCollection 2015 Sep 1.
8
A Time-Encoded Technique for fibre-based hyperspectral broadband stimulated Raman microscopy.一种用于基于光纤的高光谱宽带受激拉曼显微镜的时间编码技术。
Nat Commun. 2015 Apr 17;6:6784. doi: 10.1038/ncomms7784.
9
Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.通过体内受激拉曼散射显微镜对非洲爪蟾蝌蚪髓鞘形成进行无标记实时成像。
J Biomed Opt. 2014 Aug;19(8):086005. doi: 10.1117/1.JBO.19.8.086005.
10
Biological imaging with coherent Raman scattering microscopy: a tutorial.相干拉曼散射显微镜的生物成像：教程
J Biomed Opt. 2014 Jul;19(7):71407. doi: 10.1117/1.JBO.19.7.071407.
本文引用的文献
1
Biomolecular imaging with coherent nonlinear vibrational microscopy.基于相干非线性振动显微镜的生物分子成像
Annu Rev Phys Chem. 2013;64:77-99. doi: 10.1146/annurev-physchem-040412-110103. Epub 2012 Dec 5.
2
Raman spectroscopy and microscopy based on mechanical force detection.基于机械力检测的拉曼光谱学与显微镜技术。
Appl Phys Lett. 2011 Oct 17;99(16):161103-1611033. doi: 10.1063/1.3652760.
3
Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss.飞秒脉冲受激拉曼损耗的高灵敏度振动成像
J Phys Chem Lett. 2011 May 9;2(11):1248-1253. doi: 10.1021/jz200516n.
4
Coherent nonlinear optical imaging: beyond fluorescence microscopy.相干非线性光学成像：超越荧光显微镜
Annu Rev Phys Chem. 2011;62:507-30. doi: 10.1146/annurev.physchem.012809.103512.
5
Two-photon fluorescence excitation in continuous-wave infrared optical tweezers.连续波红外光镊中的双光子荧光激发
Opt Lett. 1995 Nov 1;20(21):2246. doi: 10.1364/ol.20.002246.
6
Stimulated Raman spectroscopy using low-power cw lasers.使用低功率连续波激光器的受激拉曼光谱学。
Opt Lett. 1977 Nov 1;1(5):152-4. doi: 10.1364/ol.1.000152.
7
Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.基于受激拉曼散射显微镜的高灵敏度无标记生物医学成像。
Science. 2008 Dec 19;322(5909):1857-61. doi: 10.1126/science.1165758.
8
Heating by absorption in the focus of an objective lens.通过在物镜焦点处吸收进行加热。
Opt Lett. 1998 Mar 1;23(5):325-7. doi: 10.1364/ol.23.000325.
9
High-sensitivity coherent anti-Stokes Raman scattering microscopy with two tightly synchronized picosecond lasers.采用两台紧密同步的皮秒激光器的高灵敏度相干反斯托克斯拉曼散射显微镜。
Opt Lett. 2002 Jul 1;27(13):1168-70. doi: 10.1364/ol.27.001168.
10
STED microscopy with continuous wave beams.连续波光束的受激发射损耗显微镜术
Nat Methods. 2007 Nov;4(11):915-8. doi: 10.1038/nmeth1108. Epub 2007 Oct 21.
Zotero 插件