D'souza Deepa, Subhas Babu G, Shetty Shishir Ram, Balan Preethi
Department of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences, Nitte University, Deralakatte, Mangalore, India.
Contemp Clin Dent. 2012 Oct;3(4):448-51. doi: 10.4103/0976-237X.107438.
Tobacco causes the generation of free radicals and reactive oxygen species (ROS) which are responsible for the high rate of lipid peroxidation. Malondialdehyde (MDA) is the most widely used agent to estimate the extent of lipid peroxidation. Timely diagnosis of the condition followed by supplementation with antioxidants like beta-carotene, pro-vitamin A, vitamin A, vitamin C, vitamin E, lipoic acid, zinc, selenium, and spirulina can prevent potentially malignant disorders.
In this study, serum MDA was measured according to the method of Buege, in 15 normal samples and 15 patients who were histopathologically diagnosed with potentially malignant disordered and they were prescribed with antioxidants for a period of 4 week-time following which potentially malignant patients serum MDA was analyzed again to determine the extent of peroxidation reactions.
The mean serum MDA level in Group C1 was 0.7900 ± 0.2336 μM/L were as the mean serum MDA level of Group S1 was 2.478 ± 0.50756 μM/L and the values between them were highly significant. The values between C1 and S2 were found to be statistically significant. The mean serum MDA of S2 was 2.160 ± 0.41252 μM/L and the values were significant when compared to S1.
Serum MDA estimation in oral pre-cancer would serve in determining the extent of lipid peroxidation. Diagnosis of patients and administration of antioxidants has proven to be effective in declining the ROS and thus reducing the extent of damage on the cells. MDA may serve as a diagnostic tool in the estimation of oral pre-cancer and in evaluation of post-treated cases.
烟草会导致自由基和活性氧（ROS）的产生，这些物质会导致脂质过氧化率升高。丙二醛（MDA）是评估脂质过氧化程度最常用的指标。及时诊断病情并补充抗氧化剂，如β-胡萝卜素、维生素A原、维生素A、维生素C、维生素E、硫辛酸、锌、硒和螺旋藻，可以预防潜在的恶性疾病。
在本研究中，根据Buege的方法测量了15份正常样本和15例经组织病理学诊断为潜在恶性疾病患者的血清MDA，并给他们开了4周的抗氧化剂，之后再次分析潜在恶性疾病患者的血清MDA，以确定过氧化反应的程度。
C1组的平均血清MDA水平为0.7900±0.2336μM/L，而S1组的平均血清MDA水平为2.478±0.50756μM/L，两组之间的值具有高度显著性。发现C1组和S2组之间的值具有统计学意义。S2组的平均血清MDA为2.160±0.41252μM/L，与S1组相比，该值具有显著性。
口腔癌前病变患者血清MDA的测定有助于确定脂质过氧化的程度。事实证明，对患者进行诊断并给予抗氧化剂可有效降低ROS，从而减少细胞损伤的程度。MDA可作为评估口腔癌前病变和治疗后病例的诊断工具。
