Neeraja Mamidi, Lakshmi Vemu, Dash P K, Parida M M, Rao P V L
Department of Microbiology, Nizam's Institute of Medical Sciences , Punjagutta Hyderabad - 500082, A.P., India .
J Clin Diagn Res. 2013 Mar;7(3):457-61. doi: 10.7860/JCDR/2013/4786.2798. Epub 2013 Mar 1.
Dengue is an acute viral infection which presents as uneventful pyrexia to a fatal complication. This infection is increasingly being recognized as the world's major emerging tropical disease and an important public health problem. This article highlights the clinical manifestations of Dengue virus infection and the various molecular tests that were used for its laboratory diagnosis.
Serum samples from 713 suspected cases of Dengue were collected between August and December 2007. The clinical profiles of 123 hospitalized patients were analyzed. Serology, RT- PCR, virus isolation and sequencing were done.
The most common clinical symptoms were fever, thrombocytopenia, rash and elevated liver enzymes. The demonstration of the Dengue RNA in 5.16% samples, the detection of Dengue specific IgM antibodies in 18% samples and the isolation of the DENV-4 and the DENV-3 viruses from the clinical samples confirmed this Dengue outbreak. A co -infection with Chikungunya was observed in 2.06% of the cases. The phylogenetic analysis revealed that the Indian Dengue-4 isolates from this outbreak belonged to the genotype I. This study clearly indicated the sudden dominance of DENV-4 in an Indian Dengue outbreak.
The surveillance of the Dengue viruses needs to be closely monitored for the emergence of newer serotype(s) in hitherto unknown areas.
登革热是一种急性病毒感染，其表现从无并发症的发热到致命的并发症不等。这种感染日益被视为世界主要的新兴热带疾病和一个重要的公共卫生问题。本文重点介绍了登革热病毒感染的临床表现以及用于实验室诊断的各种分子检测方法。
2007年8月至12月期间收集了713例疑似登革热病例的血清样本。对123例住院患者的临床资料进行了分析。进行了血清学检测、逆转录聚合酶链反应（RT-PCR）、病毒分离和测序。
最常见的临床症状为发热、血小板减少、皮疹和肝酶升高。5.16%的样本中检测到登革热RNA，18%的样本中检测到登革热特异性IgM抗体，从临床样本中分离出登革热病毒4型（DENV-4）和登革热病毒3型（DENV-3），证实了此次登革热疫情。2.06%的病例中观察到与基孔肯雅热的共同感染。系统发育分析表明，此次疫情中印度的登革热病毒4型分离株属于基因型I。这项研究清楚地表明了登革热病毒4型在印度一次登革热疫情中突然占据主导地位。
需要密切监测登革热病毒，以发现迄今未知地区出现的新型血清型。
