Facultad de Ciencias Biológicas, Instituto de Biotecnologia, Universidad Autonoma de Nuevo Leon, UANL, Monterrey, Mexico.
Int J Nanomedicine. 2013;8:1645-52. doi: 10.2147/IJN.S38708. Epub 2013 Apr 24.
Multiresistance among microorganisms to common antimicrobials has become one of the most significant concerns in modern medicine. Nanomaterials are a new alternative to successfully treat the multiresistant microorganisms. Nanostructured materials are used in many fields, including biological sciences and medicine. Recently, it was demonstrated that the bactericidal activity of zero-valent bismuth colloidal nanoparticles inhibited the growth of Streptococcus mutans; however the antimycotic potential of bismuth nanostructured derivatives has not yet been studied. The main objective of this investigation was to analyze the fungicidal activity of bismuth oxide nanoparticles against Candida albicans, and their antibiofilm capabilities. Our results showed that aqueous colloidal bismuth oxide nanoparticles displayed antimicrobial activity against C. albicans growth (reducing colony size by 85%) and a complete inhibition of biofilm formation. These results are better than those obtained with chlorhexidine, nystatin, and terbinafine, the most effective oral antiseptic and commercial antifungal agents. In this work, we also compared the antimycotic activities of bulk bismuth oxide and bismuth nitrate, the precursor metallic salt. These results suggest that bismuth oxide colloidal nanoparticles could be a very interesting candidate as a fungicidal agent to be incorporated into an oral antiseptic. Additionally, we determined the minimum inhibitory concentration for the synthesized aqueous colloidal Bi2O3 nanoparticles.
微生物对抗生素的多重耐药性已成为现代医学最关注的问题之一。纳米材料是成功治疗多重耐药微生物的新选择。纳米结构材料被用于许多领域，包括生物科学和医学。最近，已经证明零价胶体铋纳米粒子的杀菌活性抑制了变形链球菌的生长；然而，铋纳米结构衍生物的抗真菌潜力尚未得到研究。本研究的主要目的是分析氧化铋纳米粒子对白色念珠菌的杀菌活性及其抗生物膜能力。我们的结果表明，水胶体氧化铋纳米粒子对白色念珠菌的生长具有抗菌活性（使菌落大小减少 85%），并完全抑制生物膜的形成。这些结果优于氯己定、制霉菌素和特比萘芬的效果，后两者是最有效的口腔防腐剂和商业抗真菌剂。在这项工作中，我们还比较了块状氧化铋和硝酸铋（前体金属盐）的抗真菌活性。这些结果表明，氧化铋胶体纳米粒子可以作为一种很有前途的杀真菌剂候选物，被纳入口腔防腐剂中。此外，我们确定了合成水胶体 Bi2O3 纳米粒子的最小抑菌浓度。
