NUS Graduate School for Integrative Science and Engineering, National University of Singapore , Kent Ridge, Singapore 117576.
Biomacromolecules. 2013 Jun 10;14(6):2041-51. doi: 10.1021/bm400382e. Epub 2013 May 16.
Barnacle cement (BC) was utilized 'beneficially' as a surface anchor on stainless steel (SS) for coupling of functional polymer brushes via "click" reactions in both "grafting-to" and "grafting-from" processes. Ethylene sulfide (ES), propargyl carbonylimidazole (PPC) and azidoethyl carbonylimidazole (AEC) reacted with amine and/or hydroxyl groups in BC to introduce the corresponding thiol, alkyne, and azide groups on SS surfaces (SS-thiol, SS-alkyne, and SS-azide, respectively). Antifouling zwitterionic SS-PMPC surface was prepared by thiol-ene photopolymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) from the SS-thiol surface. Protein-resistant SS-PPEGMA and protein-adsorbing SS-PPFS surfaces were prepared by coupling of the respective azide-functionalized poly(poly(ethylene glycol)methyl ether methacrylate) (azido-PPEGMA) and poly(2,3,4,5,6-pentafluorostyrene) (azido-PPFS) polymer brushes in azide-alkyne "click" reaction. Antifouling alkyne-functionalized poly(N-hydroxyethyl acrylamide) (alkynyl-PHEAA) and antibacterial alkyne-functionalized poly(2-(methacryloyloxy)ethyl trimethylammonium chloride) (alkynyl-PMETA) polymer brushes were clicked on the SS-azide surface. Adsorption of bovine serum albumin and bacteria fouling of Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus epidermidis ( S. epidermidis ) were investigated on the polymer-functionalized SS surfaces. The versatile bioanchor and functional polymer brush coatings are stable in an abiotic aqueous environment for over a month.
藤壶水泥（BC）被“有益地”用作不锈钢（SS）表面的锚固剂，通过“接枝到”和“接枝自”过程中的“点击”反应来偶联功能性聚合物刷。乙硫醚（ES）、炔丙酰基羰基咪唑（PPC）和叠氮乙基羰基咪唑（AEC）与 BC 中的胺基和/或羟基反应，在 SS 表面上引入相应的硫醇、炔基和叠氮基（分别为 SS-硫醇、SS-炔基和 SS-叠氮基）。通过 SS-硫醇表面的硫醇-烯光聚合，制备了具有抗污性的两性离子 SS-PMPC 表面。通过相应的叠氮功能化聚（聚（乙二醇）甲基醚甲基丙烯酸酯）（叠氮-PPEGMA）和聚（2,3,4,5,6-五氟苯乙烯）（叠氮-PPFS）聚合物刷在叠氮-炔基“点击”反应中的偶联，制备了抗蛋白吸附的 SS-PPEGMA 和蛋白吸附的 SS-PPFS 表面。抗污炔基功能化聚（N-羟乙基丙烯酰胺）（炔基-PHEAA）和抗细菌炔基功能化聚（2-（甲基丙烯酰氧基）乙基三甲基氯化铵）（炔基-PMETA）聚合物刷被点击到 SS-叠氮表面上。在聚合物功能化 SS 表面上研究了牛血清白蛋白的吸附和革兰氏阴性大肠杆菌（E. coli）和革兰氏阳性表皮葡萄球菌（S. epidermidis）的细菌污染。在非生物水环境中，多功能生物锚固剂和功能聚合物刷涂层稳定超过一个月。
