Simon Fraser University, Engineering Science, Burnaby, British Columbia V5A 1S6, Canada.
J Biomed Opt. 2013 May;18(5):56007. doi: 10.1117/1.JBO.18.5.056007.
Small animal models of retinal diseases are important to vision research, and noninvasive high resolution in vivo rodent retinal imaging is becoming an increasingly important tool used in this field. We present a custom Fourier domain optical coherence tomography (FD-OCT) instrument for high resolution imaging of mouse retina. In order to overcome aberrations in the mouse eye, we incorporated a commercial adaptive optics system into the sample arm of the refractive FD-OCT system. Additionally, a commercially available refraction canceling lens was used to reduce lower order aberrations and specular back-reflection from the cornea. Performance of the adaptive optics (AO) system for correcting residual wavefront aberration in the mice eyes is presented. Results of AO FD-OCT images of mouse retina acquired in vivo with and without AO correction are shown as well.
视网膜疾病的小动物模型对于视觉研究非常重要，而无创、高分辨率的活体啮齿动物视网膜成像是该领域越来越重要的工具。我们展示了一种用于高分辨率小鼠视网膜成像的定制傅里叶域光学相干断层扫描（FD-OCT）仪器。为了克服小鼠眼睛的像差，我们将商业自适应光学系统集成到折射 FD-OCT 系统的样品臂中。此外，还使用了市售的折射消像差透镜来降低低阶像差和来自角膜的镜面反射。展示了自适应光学（AO）系统用于校正老鼠眼睛残留波前像差的性能。还展示了在有和没有 AO 校正的情况下，通过 AO FD-OCT 活体获取的小鼠视网膜图像的结果。
