Sookto Tularat, Srithavaj Theerathavaj, Thaweboon Sroisiri, Thaweboon Boonyanit, Shrestha Binit
Maxillofacial Prosthetic Clinic, Department of Prosthodontics, Faculty of Dentistry, Mahidol University, Thailand.
Asian Pac J Trop Biomed. 2013 May;3(5):376-80. doi: 10.1016/S2221-1691(13)60080-5.
To determine the anticandidal activities of Salvia officinalis L. (S. officinalis) essential oil against Candida albicans (C. albicans) and the inhibitory effects on the adhesion of C. albicans to polymethyl methacrylate (PMMA) resin surface.
Disc diffusion method was first used to test the anticandidal activities of the S. officinalis L. essential oil against the reference strain (ATCC 90028) and 2 clinical strains of C. albicans. Then the minimal inhibitory concentration (MIC) and minimal lethal concentration (MLC) were determined by modified membrane method. The adhesion of C. albicans to PMMA resin surface was assessed after immersion with S. officinalis L. essential oil at various concentrations of 1×MIC, 0.5×MIC and 0.25×MIC at room temperature for 30 min. One-way ANOVA was used to compare the Candida cell adhesion with the pretreatment agents and Tukey's test was used for multiple comparisons.
S. officinalis L. essential oil exhibited anticandidal activity against all strains of C. albicans with inhibition zone ranging from 40.5 mm to 19.5 mm. The MIC and MLC of the oil were determined as 2.780 g/L against all test strains. According to the effects on C. albicans adhesion to PMMA resin surface, it was found that immersion in the essential oil at concentrations of 1×MIC (2.780 g/L), 0.5×MIC (1.390 g/L) and 0.25×MIC (0.695 g/L) for 30 min significantly reduced the adhesion of all 3 test strains to PMMA resin surface in a dose dependent manner (P<0.05).
S. officinalis L. essential oil exhibited anticandidal activities against C. albicans and had inhibitory effects on the adhesion of the cells to PMMA resin surface. With further testing and development, S. officinalis essential oil may be used as an antifungal denture cleanser to prevent candidal adhesion and thus reduce the risk of candida-associated denture stomatitis.
测定鼠尾草精油对白色念珠菌的抗念珠菌活性及其对白色念珠菌黏附于聚甲基丙烯酸甲酯（PMMA）树脂表面的抑制作用。
首先采用纸片扩散法检测鼠尾草精油对白色念珠菌参考菌株（ATCC 90028）和2株临床分离株的抗念珠菌活性。然后采用改良膜法测定最低抑菌浓度（MIC）和最低致死浓度（MLC）。将白色念珠菌在室温下用1×MIC、0.5×MIC和0.25×MIC不同浓度的鼠尾草精油浸泡30分钟后，评估其对PMMA树脂表面的黏附情况。采用单因素方差分析比较念珠菌细胞黏附与预处理剂的关系，并采用Tukey检验进行多重比较。
鼠尾草精油对所有白色念珠菌菌株均表现出抗念珠菌活性，抑菌圈范围为40.5毫米至19.5毫米。该精油对所有测试菌株的MIC和MLC均确定为2.780克/升。根据对白色念珠菌黏附于PMMA树脂表面的影响，发现用1×MIC（2.780克/升）、0.5×MIC（1.390克/升）和0.25×MIC（0.695克/升）浓度的精油浸泡30分钟，能以剂量依赖方式显著降低所有3株测试菌株对PMMA树脂表面的黏附（P<0.05）。
鼠尾草精油对白色念珠菌具有抗念珠菌活性，并对细胞黏附于PMMA树脂表面有抑制作用。经过进一步测试和开发，鼠尾草精油可作为一种抗真菌义齿清洁剂，以防止念珠菌黏附，从而降低念珠菌相关性义齿性口炎的风险
