Mocan Andrei, Babota Mihai, Pop Anca, Fizeșan Ionel, Diuzheva Alina, Locatelli Marcello, Carradori Simone, Campestre Cristina, Menghini Luigi, Sisea Cristian R, Soković Marina, Zengin Gokhan, Păltinean Ramona, Badarau Sabin, C Vodnar Dan C, Crișan Gianina
Faculty of Pharmacy, "Iuliu Hațieganu" University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania.
Department of Pharmacy, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Antioxidants (Basel). 2020 Jun 2;9(6):480. doi: 10.3390/antiox9060480.
Even though genus is one of the most known and studied taxa of Lamiaceae family, the knowledge regarding the chemical composition and health-related benefits of some locally used species (mostly endemic) is still scarce. In this regard, the present work aims to evaluate the chemical profile and potential bioactivities of 70% () ethanolic extracts obtained from the less-studied and in comparison with . HPLC-PDA analysis revealed the presence of rutin and catechin as the main compounds in the extracts of the three studied species (using the employed HPLC method), whereas the presence of naringenin was highlighted only in extract. Chlorogenic acid, rutin and quercetin were identified and quantified for the first time in extracts. The in vitro antioxidant capacity of each extract was tested through complementary methods (phosphomolybdenum assay, DPPH, ABTS, CUPRAC and FRAP assays), and correlated with the presence of phenolics (especially flavonoids) in high amounts. The neuroprotective and antidiabetic abilities of (the most active as AChE, BChE and α-glucosidase inhibitor), (the most active as α-amylase inhibitor) and were also studied. For each extract it was determined the antimicrobial, antifungal and cytotoxic effects using in vitro assays. The obtained results confirm the potential of and as promising sources of bioactive compounds and as a starting point for further analyses.
尽管属是唇形科最知名且研究最多的分类群之一，但关于一些当地使用的物种（大多为特有物种）的化学成分及其对健康有益之处的知识仍然匮乏。在这方面，本研究旨在评估从研究较少的[物种名称1]和[物种名称2]中获得的70%（体积分数）乙醇提取物的化学特征和潜在生物活性，并与[物种名称3]进行比较。高效液相色谱 - 光电二极管阵列检测（HPLC - PDA）分析表明，在所研究的三种物种的提取物中（采用所使用的HPLC方法），芦丁和儿茶素是主要化合物，而仅在[物种名称1]提取物中检测到柚皮素。绿原酸、芦丁和槲皮素首次在[物种名称2]提取物中被鉴定和定量。通过互补方法（磷钼酸法、DPPH法、ABTS法、CUPRAC法和FRAP法）测试了每种提取物的体外抗氧化能力，并将其与大量酚类物质（尤其是黄酮类化合物）的存在相关联。还研究了[物种名称1]（作为乙酰胆碱酯酶、丁酰胆碱酯酶和α - 葡萄糖苷酶抑制剂活性最高）、[物种名称2]（作为α - 淀粉酶抑制剂活性最高）和[物种名称3]的神经保护和抗糖尿病能力。对于每种提取物，使用体外试验确定了其抗菌、抗真菌和细胞毒性作用。所得结果证实了[物种名称1]和[物种名称2]作为生物活性化合物的潜在来源以及作为进一步分析起点的潜力。
