Ferreira Joselena M, Fernandes-Silva Caroline C, Salatino Antonio, Negri Giuseppina, Message Dejair
Universidade de São Paulo, Instituto de Biociências, São Paulo, SP, Brazil.
Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil.
J Sci Food Agric. 2017 Aug;97(11):3552-3558. doi: 10.1002/jsfa.8210. Epub 2017 Feb 8.
Propolis is a bee product with wide diversity of biological activity. It has a complex composition, which is dependent on its botanical source. The present study aimed to determine the chemical profile, antioxidant activity and botanical origin of two samples of a propolis type from two locations of the state of Rio Grande do Norte (RN, north-east Brazil).
The standard chemical characteristics of the RN propolis are similar or superior to the internationally marketed Brazilian green propolis. RN propolis from two locations have high antioxidant activity, corresponding to 10% (municipality of Afonso Bezerra) and 13% (municipality of Alto do Rodrigues) of quercetin activity by the 2,2-diphenyl-1-picrylhydrazyl method and to 15% (both locations) by the β-carotene discoloration method. High-performance liquid chromatography with diode array detection (HPLC-DAD)-electrospray ionization-tandem mass spectrometry analyses revealed that most constituents of the RN propolis are flavonoids, mainly flavonols and chalcones. HPLC-DAD analysis of ethanol extracts revealed a great similarity between the chemical profile of RN propolis and shoot apices of 'jurema-preta' (Mimosa tenuiflora, Leguminosae, Mimosoideae).
'Jurema-preta' shoot apices are likely resin sources of RN propolis. The chemical characteristics and antioxidant property of RN propolis provide promising prospects for the introduction of this type of propolis into the apicultural market. © 2017 Society of Chemical Industry.
蜂胶是一种具有广泛生物活性的蜂产品。其成分复杂，取决于植物来源。本研究旨在确定巴西北里奥格兰德州（RN）两个地点的一种蜂胶类型的两个样品的化学特征、抗氧化活性和植物来源。
RN蜂胶的标准化学特征与国际市场上销售的巴西绿蜂胶相似或更优。来自两个地点的RN蜂胶具有较高的抗氧化活性，通过2,2-二苯基-1-苦基肼法测定，相当于槲皮素活性的10%（阿方索·贝泽拉市）和13%（阿尔托·多罗德里格斯市），通过β-胡萝卜素褪色法测定，两个地点均为15%。二极管阵列检测高效液相色谱（HPLC-DAD）-电喷雾电离串联质谱分析表明，RN蜂胶的大多数成分是黄酮类化合物，主要是黄酮醇和查耳酮。乙醇提取物的HPLC-DAD分析显示，RN蜂胶的化学特征与“黑朱雷马”（含羞草，豆科，含羞草亚科）的茎尖非常相似。
“黑朱雷马”的茎尖可能是RN蜂胶的树脂来源。RN蜂胶的化学特征和抗氧化性能为将这种蜂胶引入养蜂市场提供了广阔前景。©2017化学工业协会。
