Department of Chemistry, Indian Institute of Technology Kanpur, Kanpur, 208016, India.
Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, Gujarat, 382 011, India.
Arch Microbiol. 2023 Nov 28;206(1):6. doi: 10.1007/s00203-023-03729-z.
Paracoccus species are metabolically versatile gram-negative, aerobic facultative methylotrophic bacteria showing enormous promise for environmental and bioremediation studies. Here we report, the complete genome analysis of Paracoccus sp. strain DMF (P. DMF) that was isolated from a domestic wastewater treatment plant in Kanpur, India (26.4287 °N, 80.3891 °E) based on its ability to degrade a recalcitrant organic solvent N, N-dimethylformamide (DMF). The results reveal a genome size of 4,202,269 base pairs (bp) with a G + C content of 67.9%. The assembled genome comprises 4141 coding sequences (CDS), 46 RNA sequences, and 2 CRISPRs. Interestingly, catabolic operons related to the conventional marine-based methylated amines (MAs) degradation pathway were functionally annotated within the genome of an obligated aerobic heterotroph that is P. DMF. The genomic data-based characterization presented here for the novel heterotroph P. DMF aims to improve the understanding of the phenotypic gene products, enzymes, and pathways involved with greater emphasis on facultative methylotrophic motility-based latent pathogenicity.
副球菌属是一种代谢多样的革兰氏阴性、需氧兼性甲基营养细菌，在环境和生物修复研究方面具有巨大的应用前景。在这里，我们报告了一株副球菌属（P. DMF）的全基因组分析，该菌是从印度坎普尔的一家污水处理厂（26.4287°N，80.3891°E）中分离出来的，它具有降解难降解有机溶剂 N，N-二甲基甲酰胺（DMF）的能力。结果表明，该基因组大小为 4202269 个碱基对（bp），G+C 含量为 67.9%。组装的基因组包括 4141 个编码序列（CDS）、46 个 RNA 序列和 2 个 CRISPRs。有趣的是，在一个需氧异养生物 P. DMF 的基因组中，功能注释了与传统海洋甲基化胺（MAs）降解途径相关的分解代谢操纵子。这里基于基因组数据对新型异养生物 P. DMF 的特性进行了描述，旨在提高对表型基因产物、酶和途径的理解，重点是兼性甲基营养运动相关的潜在致病性。
