Bundesanstalt für Geowissenschaften und Rohstoffe, Hannover, Germany.
FEMS Microbiol Ecol. 2013 Sep;85(3):578-92. doi: 10.1111/1574-6941.12146. Epub 2013 May 29.
The investigated deeply buried marine sediments of the shallow shelf off New Jersey, USA, are characterized by low organic carbon content and total cell counts of < 10(7) cells per mL sediment. The qPCR data for Bacteria and Archaea were in the same orders of magnitude as the total cell counts. Archaea and Bacteria occurred in similar 16S rRNA gene copy numbers in the upper part of the sediments, but Bacteria dominated in the lowermost part of the analyzed sediment cores down to a maximum analyzed depth of c. 50 meters below seafloor (mbsf). The bacterial candidate division JS1 and the classes Anaerolineae and Caldinilineae of the Chloroflexi were almost as highly abundant as the total Bacteria. Similarly high dsrA gene copy numbers were found for sulfate reducers. The abundance of the Fe(III) and Mn(IV) reducers comprising Geobacteraceae in the upper c. 15 mbsf correlated with concentrations of manganese and iron in the pore water. The isolated 16S rRNA gene sequences of Archaea in clone libraries could be allocated to the phyla Thaumarchaeota, Euryarchaeota, and Crenarchaeota with 1%, 14%, and 85%, respectively. The typical deep subsurface sediment-associated groups MBG-B, MBG-D, MCG, and SAGMEG were represented in the sediment community. MCG was the dominant group with a high diversity of the isolated 16S rRNA gene sequences.
对美国新泽西近海浅滩深处埋藏的海洋沉积物进行了调查，这些沉积物的特点是有机碳含量低，每毫升沉积物中的总细胞数<10(7)个。细菌和古菌的 qPCR 数据与总细胞计数处于同一数量级。在沉积物的上部，古菌和细菌的 16S rRNA 基因拷贝数相似，但在分析的沉积物芯的最下部，细菌占主导地位，分析深度可达约 50 米海底以下（mbsf）。细菌候选门 JS1 和绿弯菌门的厌氧线菌纲和钙嗜热菌纲与总细菌一样丰富。硫酸盐还原菌的 dsrA 基因拷贝数也同样高。包含 Geobacteraceae 的 Fe(III)和 Mn(IV)还原剂在上部约 15 mbsf 的丰度与孔隙水中锰和铁的浓度相关。克隆文库中分离的古菌 16S rRNA 基因序列可分配到泉古菌门、广古菌门和泉古菌门，分别占 1%、14%和 85%。沉积物群落中存在典型的深地下沉积物相关的 MBG-B、MBG-D、MCG 和 SAGMEG 群体。MCG 是优势群体，其分离的 16S rRNA 基因序列多样性很高。
