Bailey D N, Bove J R
Transfusion. 1975 May-Jun;15(3):244-9. doi: 10.1046/j.1537-2995.1975.15375160359.x.
Blood was drawn from ten healthy volunteer donors into citrate-phosphate-dextrose (CPD) anticoagulant and placed on the quarantine shelf of the blood bank refrigerator. Plasma dextrose, sodium, potassium, chloride, bicarbonate, GOT, LDH, and hemoglobin as well as WBC, hematocrit, MCV, MCHC, whole blood pH, and ammonia were measured on all samples initially and at one, two, seven, 14, 21, and 28 days of storage at 4 C. Whole blood lactate also was analyzed serially on five of the units. An additional 27 units of CPD bank blood (two to 21 days of age), routinely processed, handled, and stored by the blood bank, were submitted to the same analyses on the day of administration to the patient. Five of these processed units, 21 days old, were resampled at 28 days. Results of the analyses are presented and discussed. The most pronounced changes were seen for dextrose, potassium, bicarbonate, lactate, LDH, ammonia, and hemoglobin. Plasma dextrose and bicarbonate declined in concentration while potassium, lactate, LDH, ammonia, and hemoglobin rose with storage. In general, changes in the regularly processed, singly sampled bank units were greater than those observed in the specially processed, quarantined units sampled serially. This study indicates that routine transportation, processing, and handling of bank blood may lead to increased biochemical alteration.
从十名健康志愿者捐献者采集血液，注入枸橼酸盐 - 磷酸盐 - 葡萄糖（CPD）抗凝剂中，然后放置在血库冰箱的隔离架上。对所有样本在初始时以及在4℃储存1天、2天、7天、14天、21天和28天时，测定血浆葡萄糖、钠、钾、氯、碳酸氢盐、谷草转氨酶（GOT）、乳酸脱氢酶（LDH）和血红蛋白，以及白细胞（WBC）、血细胞比容、平均红细胞体积（MCV）、平均红细胞血红蛋白浓度（MCHC）、全血pH值和氨。还对其中五个样本系列分析全血乳酸。另外27单位CPD库存血（储存2至21天），由血库常规处理、处理和储存，在输注给患者当天进行相同分析。这些处理过的单位中有五个储存21天的，在28天时重新采样。给出并讨论了分析结果。葡萄糖、钾、碳酸氢盐、乳酸、LDH、氨和血红蛋白的变化最为明显。储存时，血浆葡萄糖和碳酸氢盐浓度下降，而钾、乳酸、LDH、氨和血红蛋白升高。一般来说，常规处理、单次采样的库存血单位的变化大于特别处理、系列采样的隔离单位中观察到的变化。本研究表明，库存血的常规运输、处理和操作可能导致生化改变增加。
