Department of Engineering Science and Mechanics, The Pennsylvania State University, University Park, PA 16802, USA.
Lab Chip. 2013 Jun 21;13(12):2183-98. doi: 10.1039/c3lc90042h. Epub 2013 May 14.
Recent developments on various lab-on-a-chip techniques allow miniaturized and integrated devices to perform on-chip single-molecule studies. Fluidic-based platforms that utilize unique microscale fluidic behavior are capable of conducting single-molecule experiments with high sensitivities and throughputs, while biomolecular systems can be studied on-chip using techniques such as DNA curtains, magnetic tweezers, and solid-state nanopores. The advances of these on-chip single-molecule techniques lead to next-generation lab-on-a-chip devices, such as DNA transistors, and single-molecule real-time (SMRT) technology for rapid and low-cost whole genome DNA sequencing. In this Focus article, we will discuss some recent successes in the development of lab-on-a-chip techniques for single-molecule studies and expound our thoughts on the near future of on-chip single-molecule studies.
各种微流控芯片技术的最新进展使得微型化和集成化设备能够进行芯片上的单分子研究。基于流体的平台利用独特的微尺度流体行为，能够以高灵敏度和高通量进行单分子实验，而生物分子系统可以使用 DNA 帘、磁镊和固态纳米孔等技术在芯片上进行研究。这些芯片上单分子技术的进步带来了下一代的芯片上设备，如 DNA 晶体管和单分子实时 (SMRT) 技术，用于快速和低成本的全基因组 DNA 测序。在这篇专题文章中，我们将讨论在开发用于单分子研究的芯片技术方面的一些最新成功案例，并阐述我们对芯片上单分子研究的近期未来的看法。
