Lincoln Ventures Ltd, Christchurch, New Zealand.
Anal Bioanal Chem. 2011 May;400(4):931-45. doi: 10.1007/s00216-011-4663-6. Epub 2011 Jan 20.
Whole cell biosensors are the focus of considerable and increasing interest worldwide as methods for detecting and quantifying environmental toxicity, including biochemical oxygen demand (BOD), heavy metals, antibiotics, pesticides and herbicides. This review follows the development of whole cell biosensors from attempts to utilise changes in cellular metabolism to determine BOD and general toxicity, through the exploitation of unique metabolic pathways to detect specific toxicants, to the increasingly widespread use of genetic engineering to build new, and modify existing, sensing pathways.
作为检测和量化环境毒性的方法，全细胞生物传感器受到了全球越来越多的关注和兴趣，包括生化需氧量（BOD）、重金属、抗生素、农药和除草剂。本综述按照全细胞生物传感器的发展顺序进行介绍，从利用细胞代谢变化来测定 BOD 和一般毒性的尝试，到利用独特的代谢途径来检测特定的毒物，再到越来越广泛地利用基因工程来构建新的、修饰现有的传感途径。
