Muñoz Juan M, Coco Rosa M, Sanabria M Rosa, Cuadrado Ruben, Blanco Eduardo
Instituto de Oftalmobiologia Aplicada (IOBA), Universidad de Valladolid, Spain ; Consultorio 225, Consultorios América, Vía España, Panamá, Panama.
J Ophthalmol. 2013;2013:309192. doi: 10.1155/2013/309192. Epub 2013 Apr 22.
Purpose. To compare the autofluorescence images of the Zeiss versus Topcon eye fundus cameras and design an objective way to quantify it. Procedures. The IMAGEJ software was used to determine the gray level corresponding to the darkest veins and the peripapillary ring (thresholds), the level of white of the brightest perifoveal area, their difference (contrast level), and the suprathreshold area for each photograph. Results. Carl Zeiss has higher contrast values than Topcon. The Topcon contrast presented a crest with further decline as the suprathreshold area continued to increase. On the contrary, the Zeiss profile did not decline in contrast. Conclusions and Message. The Carl Zeiss camera showed superior contrast ability over the Topcon when performing autofluorescence imaging. We set objective parameters to compare fundus cameras FAF images. These parameters could be the base to objectively measure and determine changes and realize followup to areas of hyper- or hypofluorescence.
目的。比较蔡司(Zeiss)和拓普康(Topcon)眼底相机的自发荧光图像,并设计一种客观的方法对其进行量化。方法。使用IMAGEJ软件确定对应于最暗静脉和视乳头周围环(阈值)的灰度级、最亮的黄斑中心凹周围区域的白色水平、它们的差值(对比度水平)以及每张照片的超阈值区域。结果。卡尔·蔡司(Carl Zeiss)的对比度值高于拓普康。拓普康的对比度呈现出一个峰值,随着超阈值区域的持续增加而进一步下降。相反,蔡司的曲线对比度并未下降。结论与启示。在进行自发荧光成像时,卡尔·蔡司相机显示出比拓普康更好的对比度能力。我们设定了客观参数来比较眼底相机的自发荧光图像。这些参数可以作为客观测量和确定变化以及对高荧光或低荧光区域进行随访的基础。