ITZ Ecology & Evolution, TiHo Hannover, Bünteweg 17d, D-30559, Hannover, Germany.
Mol Ecol Resour. 2013 Nov;13(6):1069-81. doi: 10.1111/1755-0998.12125. Epub 2013 May 28.
The mitochondrial CO1 gene (cytochrome c oxidase I) is a widely accepted metazoan barcode region. In insects, the mitochondrial NADH dehydrogenase subunit 1 (ND1) gene region has proved to be another suitable marker especially for the identification of lower level taxonomic entities such as populations and sister species. To evaluate the potential of distance-based thresholds and character-based DNA barcoding for the identification of problematic species-rich taxa, both markers, CO1 and ND1, were used as test parameters in odonates. We sequenced and compared gene fragments of CO1 and ND1 for 271 odonate individuals representing 51 species, 22 genera and eight families. Our data suggests that (i) the combination of the CO1 and ND1 fragment forms a better identifier than a single region alone; and (ii) the character-based approach provides higher resolution than the distance-based method in Odonata especially in closely related taxonomic entities.
线粒体 CO1 基因（细胞色素 c 氧化酶 I）是广泛接受的后生动物条形码区域。在昆虫中，线粒体 NADH 脱氢酶亚基 1（ND1）基因区域已被证明是另一个合适的标记，特别是对于鉴定种群和姐妹种等较低分类实体。为了评估基于距离的阈值和基于特征的 DNA 条形码在鉴定问题物种丰富的分类群中的潜力，我们将 CO1 和 ND1 这两个标记物都用作蜻蜓目的测试参数。我们对代表 51 个物种、22 个属和 8 个科的 271 只蜻蜓个体的 CO1 和 ND1 基因片段进行了测序和比较。我们的数据表明：（i）CO1 和 ND1 片段的组合比单一区域的单独组合形成更好的标识符；（ii）在蜻蜓目中，基于特征的方法比基于距离的方法提供了更高的分辨率，特别是在密切相关的分类实体中。
