Koroiva Ricardo, Pepinelli Mateus, Rodrigues Marciel Elio, Roque Fabio de Oliveira, Lorenz-Lemke Aline Pedroso, Kvist Sebastian
Ecology and Conservation Graduate Program, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Laboratório de Ecologia, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
PLoS One. 2017 Aug 1;12(8):e0182283. doi: 10.1371/journal.pone.0182283. eCollection 2017.
We present a DNA barcoding study of Neotropical odonates from the Upper Plata basin, Brazil. A total of 38 species were collected in a transition region of "Cerrado" and Atlantic Forest, both regarded as biological hotspots, and 130 cytochrome c oxidase subunit I (COI) barcodes were generated for the collected specimens. The distinct gap between intraspecific (0-2%) and interspecific variation (15% and above) in COI, and resulting separation of Barcode Index Numbers (BIN), allowed for successful identification of specimens in 94% of cases. The 6% fail rate was due to a shared BIN between two separate nominal species. DNA barcoding, based on COI, thus seems to be a reliable and efficient tool for identifying Neotropical odonate specimens down to the species level. These results underscore the utility of DNA barcoding to aid specimen identification in diverse biological hotspots, areas that require urgent action regarding taxonomic surveys and biodiversity conservation.
我们展示了一项对巴西上巴拉那河流域新热带区蜻蜓目昆虫的DNA条形码研究。在被视为生物热点地区的“塞拉多”和大西洋森林的过渡区域共采集到38个物种，并为所采集的标本生成了130个细胞色素c氧化酶亚基I（COI）条形码。COI基因种内变异（0 - 2%）和种间变异（15%及以上）之间存在明显差距，由此产生的条形码索引号（BIN）分离使得94%的标本得以成功鉴定。6%的失败率是由于两个不同命名物种共享一个BIN。因此，基于COI的DNA条形码似乎是一种可靠且高效的工具，可将新热带区蜻蜓目标本鉴定到物种水平。这些结果强调了DNA条形码在协助鉴定不同生物热点地区标本方面的实用性，这些地区在分类学调查和生物多样性保护方面需要紧急行动。
