Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, People's Republic of China.
Extremophiles. 2013 Jul;17(4):663-8. doi: 10.1007/s00792-013-0549-1. Epub 2013 May 31.
A moderately halophilic bacteria designed strain NY-011(T) was isolated from the high salt culture of Dunaliella salina in Chengdu of Sichuan Province, China. The isolate was Gram-negative, nonmotile, rod-shaped and 12.5-21.6 μm in length. Colonies on solid media are circular, wet, smooth and cream. The strain grew optimally at 37 °C, pH 7.0 and in the presence of 8 % NaCl. Acid was produced from glycerol, D-arabinose, glucose, trehalose, inositol, mannose, mannitol, sucrose, maltose and sorbitol. Catalase is produced but not oxidase. The major fatty acids are C18: 1ω7c (37.59 %), C19: 0 cyclo ω8c (18.29 %), C16: 0 (16.05 %) and C6: 0 (12.43 %). The predominant respiratory lipoquinone found in strain NY-011(T) is ubiquinone with nine isoprene units (Q-9). The genomic DNA G + C content of strain NY-011(T) was 62.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NY-011(T) belonged to the genus Halomonas. The highest levels of 16S rRNA gene sequence similarity were found between the strain NY-011(T) and H. pantelleriensis (sequence similarity 98.43 %). However, the levels of DNA-DNA relatedness between them were only 23.1 %. In addition, the strain NY-011(T) had a phenotypic profile that readily distinguished it from H. pantelleriensis. The strain NY-011(T) therefore represents a new species of the genus Halomonas, for which the name Halomonas socia sp. nov. is proposed, with NY-011(T) (=CCTCC AB 2011033(T) = KCTC 23671(T)) as the type strain.
一株中度嗜盐菌 NY-011(T) 从中国四川省成都市杜氏盐藻的高盐培养物中分离得到。该分离株革兰氏阴性，无动力，杆状，长 12.5-21.6μm。固体培养基上的菌落呈圆形、湿润、光滑、奶油色。该菌株在 37°C、pH7.0 和 8%NaCl 的存在下生长最佳。能够从甘油、D-阿拉伯糖、葡萄糖、海藻糖、肌醇、甘露糖、甘露醇、蔗糖、麦芽糖和山梨醇产酸。该菌株产生过氧化氢酶但不产生氧化酶。主要脂肪酸为 C18:1ω7c（37.59%）、C19:0 环 ω8c（18.29%）、C16:0（16.05%）和 C6:0（12.43%）。在 NY-011(T) 菌株中发现的主要呼吸类脂醌是具有九个异戊二烯单位的泛醌（Q-9）。菌株 NY-011(T) 的基因组 DNA G+C 含量为 62.7mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 NY-011(T) 属于盐单胞菌属。与 NY-011(T) 菌株序列相似性最高的是 H. pantelleriensis（序列相似性为 98.43%）。然而，它们之间的 DNA-DNA 相关度水平仅为 23.1%。此外，该菌株 NY-011(T) 的表型特征使其与 H. pantelleriensis 很容易区分。因此，菌株 NY-011(T) 代表盐单胞菌属的一个新种，建议命名为 Halomonas socia sp. nov.，其模式菌株为 NY-011(T)=CCTCC AB 2011033(T)=KCTC 23671(T)。
