CIMO - Escola Superior Agrária, Instituto Politécnico de Bragança, Campus de Sta. Apolónia, Apartado 1172, 5301-855, Bragança, Portugal; REQUIMTE - Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Rua do Campo Alegre, 687, 4169-007, Porto, Portugal.
Phytother Res. 2014 Mar;28(3):437-43. doi: 10.1002/ptr.5013. Epub 2013 May 31.
Propolis is a beehive product with a very complex chemical composition, used since ancient times in several therapeutic treatments. As a contribution to the improvement of drugs against several tropical diseases caused by protozoa, we screened Portuguese propolis and its potential floral sources Populus x Canadensis and Cistus ladanifer against Plasmodium falciparum, Leishmania infantum, Trypanosoma brucei and Trypanosoma cruzi. The toxicity against MRC-5 fibroblast cells was evaluated to assess selectivity. The in vitro assays were performed following the recommendations of WHO Special Programme for Research and Training in Tropical Diseases (TDR) and revealed moderate activity, with the propolis extracts presenting the relatively highest inhibitory effect against T. brucei. Additionally, the antimicrobial activity against Staphylococcus aureus, Candida albicans, Trichophyton rubrum and Aspergillus fumigatus was also verified with the better results observed against T. rubrum. The quality of the extracts was controlled by evaluating the phenolic content and antioxidant activity. The observed biological activity variations are associated with the variable chemical composition of the propolis and the potential floral sources under study.
蜂胶是一种蜂巢产品，具有非常复杂的化学成分，自古以来就被用于多种治疗方法中。为了改进针对几种由原生动物引起的热带疾病的药物，我们对葡萄牙蜂胶及其潜在的花卉来源加拿大杨和乳香黄连木进行了筛选，以对抗疟原虫、利什曼原虫、布氏锥虫和克氏锥虫。我们评估了对 MRC-5 成纤维细胞的毒性，以评估其选择性。体外试验按照世界卫生组织热带病研究和培训特别规划署（TDR）的建议进行，结果显示具有中等活性，蜂胶提取物对布氏锥虫的抑制作用相对较高。此外，我们还验证了这些提取物对金黄色葡萄球菌、白色念珠菌、红色毛癣菌和烟曲霉的抗菌活性，对红色毛癣菌的效果最好。我们通过评估酚类含量和抗氧化活性来控制提取物的质量。观察到的生物活性变化与蜂胶和研究中的潜在花卉来源的可变化学成分有关。
