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转录组学方法在拟南芥早期胚胎中的应用。

Transcriptomics approaches in the early Arabidopsis embryo.

机构信息

Laboratory of Biochemistry, Wageningen University, The Netherlands.

出版信息

Trends Plant Sci. 2013 Sep;18(9):514-21. doi: 10.1016/j.tplants.2013.04.011. Epub 2013 May 29.

Abstract

Early plant embryogenesis condenses the fundamental processes underlying plant development into a short sequence of predictable steps. The main tissues, as well as stem cells for their post-embryonic maintenance, are specified through genetic control networks. A key question is how cell fates are instructed by unique cellular transcriptomes, and important insights have recently been gained through cell type-specific transcriptomics during post-embryonic development. However, the poor accessibility and small size of Arabidopsis (Arabidopsis thaliana) embryos have obstructed similar progress during embryogenesis. Here, we review the current situation in plant embryo transcriptomics, and discuss how the recent development of novel cell-specific analysis technologies will enable the identification of cellular transcriptomes in the early Arabidopsis embryo.

摘要

早期植物胚胎发生将植物发育的基本过程浓缩为一系列可预测的步骤。主要组织以及用于胚胎后维持的干细胞是通过遗传控制网络来指定的。一个关键问题是细胞命运如何通过独特的细胞转录组来指导,最近在胚胎后发育过程中的细胞类型特异性转录组学中获得了重要的见解。然而,拟南芥(Arabidopsis thaliana)胚胎的获取困难和体积小,阻碍了胚胎发生过程中的类似进展。在这里,我们回顾了植物胚胎转录组学的现状,并讨论了最近新型细胞特异性分析技术的发展如何使鉴定早期拟南芥胚胎中的细胞转录组成为可能。

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