Xiao Yao, Chen Jian, Chen Min, Deng Shao-Ji, Xiong Zhi-Qian, Tian Bao-Yu, Zhang Bing-Huo
College of Pharmacy and Life Science, Jiujiang University, Jiujiang, China.
College of Life Science, Fujian Normal University, Fuzhou, China.
Front Microbiol. 2022 May 12;13:861291. doi: 10.3389/fmicb.2022.861291. eCollection 2022.
In eutrophic water, attached bacteria of play an important role in the formation, development, and degradation of blooms. A novel actinobacterium, designated as JXJ CY 35, was isolated from the culture mass of FACHB-905 (Maf) collected from Lake Dianchi, Yunnan Province, China. Strain JXJ CY 35 was gram-positive, acid-fast staining, aerobic, with short rod-shaped cells, positive for catalase, and negative for oxidase. The isolate was able to grow at 10.0-36.0°C, pH 4.0-10.0, and tolerate up to 5.0% (w/v) NaCl, with optimal growth at 28°C, pH 7.0-8.0, and 0% (w/v) NaCl. Cell-wall peptidoglycan contains aspartic acid, glutamic acid, glycine, and alanine, with mannose, ribose, galactose, and arabinose as whole-cell sugars. Polar lipids consist of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), glycolipid (GL1-3), phosphoglycolipid (PGL), phosphatidylinositol (PI), and unidentified lipid (L1). The predominant menaquinone was MK-9. Major fatty acids (>10%) were Cω7c (37.0%) and Cω9c (18.9%). The complete genome sequence of strain JXJ CY 35 was 6,138,096 bp in size with a DNA G + C content of 68.3%. Based on 16S rRNA gene sequences, it has 98.2% similarity to JCM 18538. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain JXJ CY 35 and the closest five type strains JCM 18538, ATCC 700504, DSM 44476, DSM 44191, and CECT 8778 were 52.1, 20.3, 20.3, 20.6, and 19.8%, and 92.7, 75.5, 75.6, 76.0, and 75.2%, respectively. On the basis of the above taxonomic data and differences in physiological characteristics from the closely related type strain, strain JXJ CY 35 was determined to represent a novel species of genus , for which the name sp. nov., is proposed. The type strain is JXJ CY 35 (=KCTC 49379 = CGMCC 1.17501). Different inoculation dosages of the type strain JXJ CY 35 could exhibit different effects on the growth of Maf and its toxin synthesis and release. Strain JXJ CY 35 could promote the growth of Maf by providing it with available phosphorus, nitrogen, probably vitamins, and plant growth hormones.
在富营养化水体中，附着细菌在水华的形成、发展和降解过程中发挥着重要作用。从中国云南省滇池采集的鱼腥藻FACHB - 905（Maf）的培养物中分离出一株新型放线菌，命名为JXJ CY 35。菌株JXJ CY 35为革兰氏阳性，抗酸染色，好氧，细胞呈短杆状，过氧化氢酶阳性，氧化酶阴性。该菌株能够在10.0 - 36.0°C、pH 4.0 - 10.0的条件下生长，并能耐受高达5.0%（w/v）的NaCl，最适生长温度为28°C，pH 7.0 - 8.0，NaCl浓度为0%（w/v）。细胞壁肽聚糖含有天冬氨酸、谷氨酸、甘氨酸和丙氨酸，全细胞糖包括甘露糖、核糖、半乳糖和阿拉伯糖。极性脂类由双磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、糖脂（GL1 - 3）、磷酸糖脂（PGL）、磷脂酰肌醇（PI）和未知脂类（L1）组成。主要的甲基萘醌是MK - 9。主要脂肪酸（>10%）为Cω7c（37.0%）和Cω9c（18.9%）。菌株JXJ CY 35的完整基因组序列大小为6,138,096 bp，DNA G + C含量为68.3%。基于16S rRNA基因序列，它与JCM 18538的相似性为98.2%。菌株JXJ CY 35与最接近的五个模式菌株JCM 18538、ATCC 700504、DSM 44476、DSM 44191和CECT 8778之间的数字DNA - DNA杂交（dDDH）值和平均核苷酸同一性（ANI）值分别为52.1%、20.3%、20.3%、20.6%和19.8%，以及92.7%、75.5%、75.6%、76.0%和75.2%。根据上述分类学数据以及与密切相关模式菌株生理特征的差异，确定菌株JXJ CY 35代表一个新的属种，为此提出新物种名sp. nov.。模式菌株为JXJ CY 35（=KCTC 49379 = CGMCC 1.17501）。模式菌株JXJ CY 35的不同接种剂量对Maf的生长及其毒素合成和释放可能表现出不同的影响。菌株JXJ CY 35可以通过为Maf提供有效磷、氮，可能还有维生素和植物生长激素来促进其生长。
