Keller Heiko, Worch Remigiusz, Schwille Petra
BIOTEC, Dresden University of Technology, Dresden, Germany.
Methods Mol Biol. 2013;1008:417-38. doi: 10.1007/978-1-62703-398-5_15.
The context of the membrane is crucial for the interaction of many membrane proteins with their ligands. However, many detailed studies cannot be carried out in living cells. Therefore, studying these interactions requires model membrane systems that are compatible with the used analytical method. A big variety of these methods is available, each of which has its advantages and disadvantages. This chapter gives an overview over the existing techniques, a basic introduction into work with lipids, and detailed protocols for selected methods.
膜的环境对于许多膜蛋白与其配体的相互作用至关重要。然而，许多详细的研究无法在活细胞中进行。因此，研究这些相互作用需要与所使用的分析方法兼容的模型膜系统。有各种各样的这些方法可供使用，每种方法都有其优缺点。本章概述了现有技术，对脂质研究进行了基本介绍，并给出了所选方法的详细方案。
