Loose Martin, Schwille Petra
Dresden University of Technology, Tatzberg, Germany.
J Struct Biol. 2009 Oct;168(1):143-51. doi: 10.1016/j.jsb.2009.03.016. Epub 2009 Apr 5.
During many cellular processes such as cell division, polarization and motility, the plasma membrane does not only represent a passive physical barrier, but also provides a highly dynamic platform for the interplay between lipids, membrane binding proteins and cytoskeletal elements. Even though many regulators of these interactions are known, their mutual interdependence appears to be highly complex and difficult to study in a living cell. Over the past few years, in vitro studies on membrane-cytoskeleton interactions using biomimetic membranes turned out to be extremely helpful to get better mechanistic insight into the dynamics of these processes. In this review, we discuss some of the recent developments using in vitro assays to dissect the role of the players involved: lipids in the membrane, proteins binding to membranes and proteins binding to membrane proteins. We also summarize advantages and disadvantages of supported lipid bilayers as model membrane.
在许多细胞过程中，如细胞分裂、极化和运动，质膜不仅是一个被动的物理屏障，还为脂质、膜结合蛋白和细胞骨架成分之间的相互作用提供了一个高度动态的平台。尽管已知许多这些相互作用的调节因子，但其相互依赖性似乎高度复杂，难以在活细胞中进行研究。在过去几年中，使用仿生膜对膜 - 细胞骨架相互作用进行的体外研究被证明对更好地深入了解这些过程的动力学机制非常有帮助。在这篇综述中，我们讨论了一些利用体外实验来剖析相关参与者作用的最新进展：膜中的脂质、与膜结合的蛋白以及与膜蛋白结合的蛋白。我们还总结了支持脂质双层作为模型膜的优缺点。
