Porsius J C, van Vliet H J, van Zeijl J H, Goessens W H, Michel M F
Department of Clinical Microbiology, Erasmus University, Rotterdam, The Netherlands.
Eur J Clin Microbiol Infect Dis. 1990 May;9(5):352-5. doi: 10.1007/BF01973744.
Tests to detect circulating antibodies to Candida albicans antigens were performed in sera from 27 immunocompetent patients, 15 of whom had deep-seated candidiasis and 12 of whom were colonised by Candida albicans. For the diagnosis of deep-seated candidiasis in patients with either deep-seated candidiasis or Candida albicans colonisation, counterimmunoelectrophoresis had a sensitivity of 87% and a specificity of 75%. Using immunoblotting it could be shown that antibodies to 35K, 47K, 68K and 88K antigens of Candida albicans occurred more frequently in patients with deep-seated candidiasis than in colonised patients. The presence of dense bands in immunoblots representing antibodies against the 47K and/or 68K antigen served to discriminate significantly between deep-seated and superficial candidiasis (p less than 0.05).
对27名免疫功能正常患者的血清进行了检测，以检测针对白色念珠菌抗原的循环抗体，其中15人患有深部念珠菌病，12人被白色念珠菌定植。对于患有深部念珠菌病或白色念珠菌定植的患者，深部念珠菌病的诊断中，对流免疫电泳的敏感性为87%，特异性为75%。使用免疫印迹法可以表明，深部念珠菌病患者中针对白色念珠菌35K、47K、68K和88K抗原的抗体比定植患者中更频繁出现。免疫印迹中代表针对47K和/或68K抗原抗体的密集条带的存在有助于显著区分深部和浅表念珠菌病（p小于0.05）。
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