Park Kwanjun, Kim June Hoan, Kong Taedong, Sun Woong, Lee Jonghwan, Yang Taeseok Daniel, Choi Youngwoon
Department of Bioengineering, Korea University, Seoul 02841, South Korea.
Department of Anatomy, College of Medicine, Korea University, Seoul 02841, South Korea.
Biomed Opt Express. 2020 Aug 11;11(9):4976-4988. doi: 10.1364/BOE.399428. eCollection 2020 Sep 1.
We report a label-free imaging method for microendoscopy that uses a needle-type imaging probe. We inserted a thin GRIN lens that had been attached to a fiber bundle into a medical-grade needle that was used as an imaging probe. The introduction of the needle probe into biological tissue allows for direct access to deep regions that we otherwise could not achieve because of the multiple light scattering. To minimize invasiveness, we introduced the illuminating probe on the tissue surface, using an oblique back-illumination configuration. We achieved three-dimensional depth imaging by changing the depth of penetration. Since only the imaging probe goes deep into the tissue while leaving the illumination channels outside, the achievable signal depends on the location of the illumination channels. We explored this point and investigated the optimal condition for the illumination distance in a systematic way. We also applied this method to , as well as , imaging of a mouse brain, and confirmed that we had visualized the microvasculature embedded deep within the brain.
我们报告了一种用于显微内窥镜检查的无标记成像方法，该方法使用针型成像探头。我们将附着在纤维束上的薄梯度折射率透镜插入用作成像探头的医用级针中。将针型探头引入生物组织可直接进入深部区域，否则由于多次光散射，我们无法到达这些区域。为了将侵入性降至最低，我们采用斜后照明配置在组织表面引入照明探头。我们通过改变穿透深度实现了三维深度成像。由于只有成像探头深入组织，而照明通道留在外部，因此可实现的信号取决于照明通道的位置。我们探讨了这一点，并系统地研究了照明距离的最佳条件。我们还将此方法应用于小鼠大脑成像，证实我们已经可视化了深埋在大脑中的微血管系统。
