International Center for Genetic Engineering and Biotechnology; Aruna Asaf Ali Marg; New Delhi, India; Department of Biology and Biotechnology "L. Spallanzani"; University of Pavia; Pavia, Italy.
Plant Signal Behav. 2013 Aug;8(8). doi: 10.4161/psb.25128. Epub 2013 Jul 8.
Ionizing radiation currently represents an important tool to generate genetic variability that does not exist in nature, especially in plants. Even so, the radiological protection still represents a subject of regulatory concern. In plants, few reports dealing with the effects of γ-rays, in terms of dose rate (rate of energy deposition) and total dose (energy absorbed per unit mass), are available. In addition, plants are known to be more radioresistant than animals. The use of ionizing radiations for studying various aspects of transcription regulation may help elucidate some of the unanswered questions regarding DNA repair in plants. Under these premises, microRNAs have emerged as molecules involved in gene regulation in response to various environmental conditions as well as in other aspects of plant development. Currently, no report on the changes in microRNAs expression patterns in response to γ-ray treatments exists in plants, even if this subject is extensively studies in human cells. The present study deals with the expression profiles of three miRNAs, namely osa-miR414, osa-miR164e and osa-miR408 and their targeted helicase genes (OsABP, OsDBH and OsDSHCT) in response to different doses of γ-rays delivered both at low and high dose rates. The irradiated rice seeds were grown both in the presence of water and 100 mM NaCl solution. DNA damage and reactive species accumulation were registered, but no dose- or time-dependent expression was observed in response to these treatments.
电离辐射目前是产生自然界中不存在的遗传变异的重要工具，尤其是在植物中。即便如此，辐射防护仍然是一个受到监管关注的主题。在植物中，关于 γ 射线的影响的报告很少涉及剂量率（能量沉积率）和总剂量（单位质量吸收的能量）。此外，众所周知，植物比动物更具有放射性抗性。利用电离辐射研究转录调控的各个方面可能有助于阐明植物 DNA 修复方面的一些未解决的问题。在此前提下，microRNAs 已成为参与基因调控的分子，以响应各种环境条件以及植物发育的其他方面。目前，即使在人类细胞中对此进行了广泛的研究，但在植物中，尚未有关于 microRNAs 表达模式对 γ 射线处理的响应的报告。本研究探讨了在低剂量率和高剂量率下接受不同剂量 γ 射线处理后，三种 microRNAs（osa-miR414、osa-miR164e 和 osa-miR408）及其靶向解旋酶基因（OsABP、OsDBH 和 OsDSHCT）的表达谱。对辐照的水稻种子进行了在有和没有 100mM NaCl 溶液的情况下的生长实验。记录了 DNA 损伤和活性物质的积累，但没有观察到这些处理的剂量或时间依赖性表达。
