Microbiología, Departamento de Biología, Universidad de las Islas Baleares, 07122, Palma de Mallorca, Islas Baleares, Spain.
Microb Ecol. 2013 Oct;66(3):522-32. doi: 10.1007/s00248-013-0246-8. Epub 2013 Jun 4.
Pseudomonas stutzeri is a widely distributed species with very high genetic diversity and metabolic capacities, occupying many diverse ecological niches. A collection of 229 P. stutzeri strains isolated from different habitats and geographical locations has been previously characterised phylogenetically by rpoD gene sequencing analysis and in the present study 172 of them phenotypically by whole-cell MALDI-TOF mass spectrometry. Fifty-five strains were further analysed by multilocus sequencing analysis to determine the phylogenetic population structure. Both methods showed coherence in strain grouping; 226 strains were allocated in the 18 genomovars known presently. The remaining three strains are proposed as references for three novel genomovars in the species. The correlation and usefulness of sequence-based phylogenetic analysis and whole-cell MALDI-TOF mass spectrometry, which are essential for autoecological studies in microbial ecology, is discussed for the differentiation of P. stutzeri populations.
施氏假单胞菌是一种分布广泛的物种，具有非常高的遗传多样性和代谢能力，占据了许多不同的生态位。先前通过 rpoD 基因测序分析对来自不同生境和地理位置的 229 株施氏假单胞菌菌株进行了系统发育特征描述，在本研究中，通过全细胞 MALDI-TOF 质谱法对其中的 172 株进行了表型分析。对 55 株进一步进行了多位点测序分析，以确定系统发育种群结构。这两种方法在菌株分组上具有一致性；226 株被分配到目前已知的 18 个基因组变种中。其余 3 株被提议作为该物种 3 个新基因组变种的参考。讨论了基于序列的系统发育分析和全细胞 MALDI-TOF 质谱法的相关性和实用性，这对于微生物生态学中的自动生态学研究是必不可少的，用于区分施氏假单胞菌种群。
