Microbiologia, Departament de Biologia, Universitat de les Illes Balears, 07122 Palma de Mallorca, Illes Balears, Spain.
Syst Appl Microbiol. 2011 Sep;34(6):408-13. doi: 10.1016/j.syapm.2011.06.001. Epub 2011 Jul 23.
Three ceftazidime-resistant strains isolated from the sewage water of a municipal hospital in Palma de Mallorca, Spain, were analysed phenotypically and genotypically to clarify their taxonomic positions. Sequence determinations and phylogenetic analyses of the 16S rRNA genes indicated that strains CS20.3(T), CS39 and CS41 were affiliated with the species of the alphaproteobacterial genus Brevundimonas, most closely related to B. bullata, B. diminuta, B. naejangsanensis and B. terrae. Additional sequences analyses of the ITS1 region of the rRNA operon and the genes for the housekeeping enzymes DNA gyrase β-subunit and RNA polymerase β-subunit, genomic DNA-DNA hybridisation similarities, cell fatty acid profiles and physiological and biochemical characterizations supported the recognition of CS20.3(T) (CCUG 58127(T)=CECT 7729(T)) as a distinct and novel species, for which the name Brevundimonas faecalis sp. nov. is proposed. Strains CS39 and CS41 were ascribed to the species B. diminuta.
从西班牙帕尔马德马略卡市一家市立医院的污水中分离出的 3 株耐头孢他啶的菌株，通过表型和基因型分析来明确其分类地位。16S rRNA 基因的序列测定和系统发育分析表明，菌株 CS20.3(T)、CS39 和 CS41 与α变形菌属的短稳杆菌属有关，与 B. bullata、B. diminuta、B. naejangsanensis 和 B. terrae 最为密切相关。rRNA 操纵子的 ITS1 区、管家酶 DNA 回旋酶β亚基和 RNA 聚合酶β亚基的基因、基因组 DNA-DNA 杂交相似性、细胞脂肪酸图谱以及生理生化特征的进一步序列分析，支持 CS20.3(T)(CCUG 58127(T)=CECT 7729(T))作为一个独特的新种，建议命名为粪短稳杆菌（Brevundimonas faecalis）sp. nov.。菌株 CS39 和 CS41 被归入 B. diminuta 种。
