Material and Chemical Research Laboratories, Industrial Technology Research Institute, Hsinchu 31040, Taiwan.
Nanotechnology. 2013 Jul 5;24(26):265101. doi: 10.1088/0957-4484/24/26/265101. Epub 2013 Jun 3.
Identification of the effects of different textured substrates on zebrafish (Danio rerio) embryos provides insights into the influence of external stimuli on normal cardiovascular functions in the developmental stages of the embryos. This knowledge can be used in numerous genetic studies using zebrafish as an animal model as well as in bioanalytical assays using digital microfluidics. In this study, zebrafish embryos were systematically positioned and in vivo imaged on four types of silicon substrates. These substrates exhibited surface textures and surface wettability that were well modulated by wet chemical etching. The heart rate of the developing embryos significantly increased by 9.1% upon exposure to textured Si substrates with nanostructured surfaces compared with bare Si substrates. Modulation of surface wettability in the tested substrates also responded to the increase in the heart rate of the embryo; however, the effect of surface wettability on heart rate was slight compared with the effect of texture. In-depth experimental and statistical investigations of heart rate under the effects of substrate textures imply a pathway through which the inner mass of the embryo reacts to external stimuli. These findings contribute to zebrafish-related studies and suggest other factors to consider in the design of nanostructure-based microfluidics and other biomedical devices.
鉴定不同质地基底对斑马鱼（Danio rerio）胚胎的影响，有助于了解胚胎发育阶段外部刺激对正常心血管功能的影响。这项知识可用于许多以斑马鱼为动物模型的遗传研究，以及使用数字微流控技术的生物分析测定。在这项研究中，我们系统性地将斑马鱼胚胎放置在四种硅质基底上，并对其进行体内成像。这些基底的表面纹理和润湿性可通过湿化学刻蚀进行很好的调控。与裸露的硅基底相比，暴露于具有纳米结构表面的纹理化 Si 基底的发育胚胎的心率显著增加了 9.1%。所测试基底的润湿性调节也响应胚胎心率的增加；然而，与纹理的影响相比，表面润湿性对心率的影响较小。对基底纹理影响下的心率进行深入的实验和统计研究，暗示了胚胎内部物质对外界刺激的反应途径。这些发现有助于斑马鱼相关研究，并建议在设计基于纳米结构的微流控和其他生物医学设备时考虑其他因素。
