Mallard Creek Polymers, Inc., 14700 Mallard Creek Road, Charlotte, NC 28262, USA.
FEBS Lett. 2013 Jul 11;587(14):2094-8. doi: 10.1016/j.febslet.2013.05.051. Epub 2013 Jun 2.
We consider biomacromolecular crowding within the cytoplasm of prokaryotic cells as a two-phase system of 'supercrowded' cytogel and 'dilute' cytosol; we simplify and quantify this model for a coccoid cell over a wide range of biomacromolecular crowding. The key result shows that the supercrowded cytogel extends the vectorial character of the plasma membrane deeper into the cytoplasm by about 20-70 nm. We discuss useful physiological insights that this model gives into the functioning of a prokaryotic cell on the micrometer scale.
我们将原核细胞细胞质中的生物大分子拥挤视为“超拥挤”细胞凝胶和“稀释”胞质溶胶的两相系统;我们简化并量化了这个模型,以适用于广泛的生物大分子拥挤范围内的球形细胞。关键结果表明,超拥挤的细胞凝胶通过大约 20-70nm 将质膜的向性特征延伸到细胞质深处。我们讨论了这个模型在微米尺度上对原核细胞功能的有用生理见解。