Reproductive Medicine Center, the People's Hospital of Zhengzhou University; Henan Provincial People's Hospital, Henan, China.
J Assist Reprod Genet. 2013 Jul;30(7):933-8. doi: 10.1007/s10815-013-0021-2. Epub 2013 Jun 6.
Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM).
Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10(-2), 1, 10(2), 10(4), 10(6) nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture.
The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P < 0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P < 0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (10(6) nM).
The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.
牛和猪的研究表明，褪黑素（MT）可以诱导不成熟卵母细胞在体外进行减数分裂成熟。本研究的目的是研究 MT 是否可以改善体外成熟（IVM）中人类卵母细胞的成熟。
将 278 个生发泡（GV）卵和 451 个第一次减数分裂中期（MI）卵进行玻璃化冷冻，解冻后进行体外成熟。所有卵均随机分配到 6 个组中，其中卵在添加不同浓度 MT（0、10(-2)、1、10(2)、10(4)、10(6) nM）的培养基中培养，并在培养的 6、12、18、24 和 48 小时评估核成熟。
GV 和 MI 卵的最佳 MT 浓度均为 1 nM。在 24 小时的培养中，在 1 nM MT 培养基中培养的 MI 卵的核成熟率明显高于其他组（P<0.05）；在 1 nM MT 培养基中培养的 GV 卵的核成熟率也明显高于对照组（P<0.05）。另一方面，在高 MT 浓度组（10(6) nM）观察到核成熟率降低。
本研究表明，低浓度外源性 MT 可改善体外成熟中人类卵母细胞的核成熟，而高浓度 MT 则呈现负面影响。
