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采用超高效液相色谱-四极杆/飞行时间质谱法研究黄酒对大鼠尿液中草药主要成分代谢物的影响：以山茱萸为例。

Investigation of the Effect of Rice Wine on the Metabolites of the Main Components of Herbal Medicine in Rat Urine by Ultrahigh-Performance Liquid Chromatography-Quadrupole/Time-of-Flight Mass Spectrometry: A Case Study on Cornus officinalis.

机构信息

Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China ; National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China.

出版信息

Evid Based Complement Alternat Med. 2013;2013:306712. doi: 10.1155/2013/306712. Epub 2013 Apr 29.

DOI:10.1155/2013/306712
PMID:23737825
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3657418/
Abstract

Ultrahigh-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-QTOF/MS) was developed for rapid and sensitive analysis of the effect of rice wine on the metabolites of the main components of herbal medicine in rat urine. Using Cornus officinalis as a model of herbal medicine, the metabolite profiles of crude and processed (steaming the crude drug presteeped in rice wine) Cornus officinalis extracts in rat urine were investigated. The metabolites of Cornus officinalis were identified by using dynamic adjustment of the fragmentor voltage to produce structure-relevant fragment ions. In this work, we identified the parent compounds and metabolites of crude and processed Cornus officinalis in rats. In total, three parent compounds and seventeen new metabolites of Cornus officinalis were found in rats. The contents of the parent compounds and metabolites in vivo varied significantly after intragastric (i.g.) administration of aqueous extracts of crude and processed Cornus officinalis. Data from this study suggests that UPLC-QTOF/MS could be used as a potential tool for uncovering the effects of excipients found in the metabolites of the main components of herbal medicine, in vivo, to predict and discover the processing mechanisms of herbal medicine.

摘要

建立了超高效液相色谱-四极杆/飞行时间质谱（UPLC-QTOF/MS）法，用于快速灵敏地分析黄酒对草药主要成分在大鼠尿液中代谢物的影响。以山茱萸为草药模型，考察了生品及（生药用黄酒预浸泡后蒸制）炮制品山茱萸提取物在大鼠尿液中的代谢物谱。采用动态调整碎片电压的方法产生结构相关的碎片离子，鉴定山茱萸的代谢物。本工作鉴定了大鼠中未加工和加工山茱萸的母体化合物和代谢物。共鉴定出大鼠中未加工和加工山茱萸的三种母体化合物和十七种新代谢物。灌胃给予生品及炮制品山茱萸水提物后，体内母体化合物和代谢物的含量差异显著。本研究表明，UPLC-QTOF/MS 可用于揭示草药主要成分代谢物中辅料的体内影响，用于预测和发现草药的炮制机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/f10a5989022b/ECAM2013-306712.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/50438851480e/ECAM2013-306712.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/674ecd2c5c66/ECAM2013-306712.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/c99597d47a1b/ECAM2013-306712.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/4f25a3af5fc3/ECAM2013-306712.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/f10a5989022b/ECAM2013-306712.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/50438851480e/ECAM2013-306712.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/674ecd2c5c66/ECAM2013-306712.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/c99597d47a1b/ECAM2013-306712.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/4f25a3af5fc3/ECAM2013-306712.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f7c/3657418/f10a5989022b/ECAM2013-306712.005.jpg

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