Presta Michele Antoniuk, Bruyneel Ben, Zanella Renato, Kool Jeroen, Krabbe Johannes G, Lingeman Henk
Chromatographia. 2009 Jun;69(Suppl 2):167-173. doi: 10.1365/s10337-009-1132-x. Epub 2009 May 26.
Turbulent-flow chromatography (TFC) on-line coupled to liquid chromatography mass spectrometry (LC-MS) is used to determine flavonoids and resveratrol in different types of wines. A fully automated system was developed in which 10 mL of sample (diluted wine) was passed over a TFC column, after which the retained analytes were separated by reversed-phase LC and detected by negative ion mode atmospheric-pressure chemical ionization (APCI) MS. The method proved to be fast, non-laborious, robust and sensitive. The feasibility of the method was tested on several red, white and rose wines. Quantitation of resveratrol was possible using the standard addition procedure. Red wine showed the highest amount of resveratrol (4 mg L(-1)), while rose and white wine contained concentrations which were about ten fold lower.
将湍流色谱法（TFC）与液相色谱-质谱联用（LC-MS）在线联用，用于测定不同类型葡萄酒中的黄酮类化合物和白藜芦醇。开发了一种全自动系统，将10 mL样品（稀释后的葡萄酒）通过TFC柱，之后保留的分析物通过反相液相色谱分离，并采用负离子模式大气压化学电离（APCI）质谱进行检测。该方法被证明快速、简便、稳健且灵敏。在几种红葡萄酒、白葡萄酒和桃红葡萄酒上测试了该方法的可行性。采用标准加入法对白藜芦醇进行定量。红葡萄酒中白藜芦醇含量最高（4 mg L⁻¹），而桃红葡萄酒和白葡萄酒中的含量约低十倍。
