Universidade Federal Fluminense, Laboratório de Diagnóstico Virológico, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Niterói, RJ, Brasil.
Braz J Med Biol Res. 2013 Jun;46(6):533-8. doi: 10.1590/1414-431X20132519. Epub 2013 May 24.
Cervical cancer is a major source of illness and death among women worldwide and genital infection with oncogenic human papillomavirus (HPV) its principal cause. There is evidence of the influence of the male factor in the development of cervical neoplasia. Nevertheless, the pathogenic processes of HPV in men are still poorly understood. It has been observed that different HPV types can be found among couples. The objective of the present study was to investigate HPV infections in female patients (n = 60 females/group) as well as in their sexual partners and to identify the concordance of HPV genotypes among them. By using the polymerase chain reaction, we detected a 95% prevalence of HPV DNA in women with cervical intraepithelial neoplasia (CIN) compared to 18.3% in women with normal cervical epithelium, with a statistically significant difference (P < 0.001). The HPV DNA prevalence was 50% in male partners of women with CIN and 16.6% in partners of healthy women. In the control group (healthy women), only 9 couples were simultaneously infected with HPV, and only 22.2% of them had the same virus type, showing a weak agreement rate (kappa index = 0.2). Finally, we observed that HPV DNA was present in both partners in 30 couples if the women had CIN, and among them, 53.3% shared the same HPV type, showing moderate agreement, with a kappa index of 0.5. This finding supports the idea of circulation and recirculation of HPV among couples, perpetuating HPV in the sexually active population, rather than true recurrences of latent infections.
宫颈癌是全球女性疾病和死亡的主要原因之一，其主要病因是生殖道感染致癌型人乳头瘤病毒（HPV）。有证据表明男性因素在宫颈癌前病变的发展中起作用。然而，男性 HPV 的致病过程仍知之甚少。已经观察到在夫妻之间可以发现不同的 HPV 类型。本研究的目的是调查女性患者（每组 60 名女性）及其性伴侣中的 HPV 感染情况，并确定它们之间 HPV 基因型的一致性。通过聚合酶链反应，我们发现患有宫颈上皮内瘤变（CIN）的女性 HPV DNA 的阳性率为 95%，而宫颈上皮正常的女性为 18.3%，差异具有统计学意义（P<0.001）。患有 CIN 的女性的男性伴侣中 HPV DNA 的阳性率为 50%，健康女性的伴侣中为 16.6%。在对照组（健康女性）中，只有 9 对夫妇同时感染 HPV，且只有 22.2%的夫妇具有相同的病毒类型，显示出较弱的一致性（kappa 指数=0.2）。最后，我们观察到如果女性患有 CIN，则 30 对夫妇中有 30 对夫妇的双方均存在 HPV DNA，其中 53.3%的夫妇共享相同的 HPV 类型，显示出中度一致性，kappa 指数为 0.5。这一发现支持 HPV 在夫妻之间循环和再循环的观点，从而使 HPV 在性活跃人群中持续存在，而不是潜伏感染的真正复发。
