Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University in Prague and University Hospital Motol, V Úvalu 84, 150 06, Prague 5, Czech Republic,
Eur Arch Otorhinolaryngol. 2014 Feb;271(2):399-405. doi: 10.1007/s00405-013-2574-1. Epub 2013 Jun 7.
Helicobacter pylori (HP) is considered a major gastric pathogen with oncogenic potential. The aim of this study was to determine whether HP is present in oropharyngeal lymphoid tissue and whether oropharyngeal HP strains carry virulence factor genes known to be involved in gastric carcinogenesis. The study included 104 subjects (41 patients with tonsillar carcinoma, 38 with chronic tonsillitis and 25 with obstructive sleep apnoea syndrome--OSAS). Detection of specific serum anti-HP antibodies was performed with an ELISA. The presence of HP in tissue was determined by culture and real-time PCR. Detection of virulence factors genes was also performed. Specific antibodies were found in 78.05% of tumour cases, 34.21% of chronic tonsillitis cases, and 72.0% of OSAS cases. The presence of HP in the tissue was detected in 73.91% of tonsillar tumours, 70.0% of tonsillitis cases, and 69.23% of OSAS specimens. The results of the virulence factor gene analysis showed the majority of the s1b (52.4%) and m2 (59.5%) alleles of vacA gene and limited abundance of cagA gene (12.5%). Results confirm that HP may colonise oropharyngeal lymphoid tissue. Oropharyngeal HP colonisation was frequently found in the oropharyngeal cancer group and in patients with benign oropharyngeal diseases. A virulence factor gene analysis showed differences from the predominant strains most commonly found in the stomach. The strains obtained from the oropharynx differed primarily by the lower abundance of the cagA gene and carried the less virulent vacA gene allele combination.
幽门螺杆菌（HP）被认为是一种具有致癌潜能的主要胃病原体。本研究旨在确定 HP 是否存在于咽淋巴组织中，以及咽 HP 株是否携带已知与胃致癌作用相关的毒力因子基因。该研究纳入了 104 例受试者（41 例扁桃体癌患者、38 例慢性扁桃体炎患者和 25 例阻塞性睡眠呼吸暂停综合征（OSAS）患者）。采用 ELISA 法检测特异性血清抗 HP 抗体。通过培养和实时 PCR 检测组织中 HP 的存在。还检测了毒力因子基因的存在。在 78.05%的肿瘤病例、34.21%的慢性扁桃体炎病例和 72.0%的 OSAS 病例中发现了特异性抗体。在 73.91%的扁桃体肿瘤、70.0%的慢性扁桃体炎病例和 69.23%的 OSAS 标本中检测到组织中存在 HP。毒力因子基因分析的结果显示，vacA 基因的 s1b（52.4%）和 m2（59.5%）等位基因以及 cagA 基因的丰度有限（12.5%）。结果证实 HP 可能定植于咽淋巴组织。咽 HP 定植在咽癌组和良性咽疾病患者中较为常见。毒力因子基因分析显示，与胃中最常见的主要菌株相比存在差异。从咽中获得的菌株主要区别在于 cagA 基因的丰度较低，携带毒力较弱的 vacA 基因等位基因组合。
