Grassland and Forages Research Center, National Institute of Animal Science, Cheonan, Republic of Korea.
Pharm Biol. 2013 Aug;51(8):1066-76. doi: 10.3109/13880209.2013.768274. Epub 2013 Jun 7.
Recently, there has been renewed interest in barley (Hordeum vulgare L. Poaceae) as a functional food and for its medicinal properties.
This study examines the anti-inflammatory potential of the active fractions of barley and the mechanisms involved.
The macrophages were exposed to 100 μg/mL of each of the barley extracts in the presence of 1 μg/mL lipopolysaccharide (LPS) and after 24 or 48 h of incubation, cells or culture supernatants were analyzed by various assays. The anti-inflammatory potential of barley fractions was also investigated using the LPS-injected septic mouse model. The active constituents in the fractions were identified using gas chromatography-mass spectrometry (GC-MS).
The active fractions, named F₄, F₇, F₉ and F₁₂, inhibited almost completely the LPS-induced production of nitric oxide (NO) and inducible NO synthase. Pre-treatment with these fractions at 100 μg/mL diminished the tumor necrosis factor-α (TNF-α) levels to 19.8, 3.5, 1.2 and 1.7 ng/mL, respectively, compared to LPS treatment alone (41.5 ng/mL). These fractions at 100 μg/mL also suppressed apparently the secretion of interleukin (IL)-6 and IL-1β and the DNA-binding activity of nuclear factor-κB in LPS-stimulated cells. Mice injected intraperitoneally with LPS (30 mg/kg BW) showed 20% survival at 48 h after injection, whereas oral administration of the fractions improved the survival rates to 80%. GC-MS analysis revealed the presence of the derivatives of benzoic and cinnamic acids and fatty acids in the fractions.
The aerial parts of barley are useful as functional food to prevent acute inflammatory responses.
最近，人们对大麦（Hordeum vulgare L. Poaceae）作为功能性食品及其药用特性重新产生了兴趣。
本研究检查了大麦活性部位的抗炎潜力及其涉及的机制。
将巨噬细胞暴露于 100μg/mL 的每种大麦提取物中，同时存在 1μg/mL 的脂多糖（LPS），在 24 或 48 小时孵育后，通过各种测定法分析细胞或培养上清液。还使用 LPS 注射的脓毒症小鼠模型研究了大麦馏分的抗炎潜力。使用气相色谱-质谱（GC-MS）鉴定馏分中的活性成分。
命名为 F₄、F₇、F₉ 和 F₁₂ 的活性馏分几乎完全抑制了 LPS 诱导的一氧化氮（NO）和诱导型一氧化氮合酶的产生。与单独 LPS 处理相比，这些馏分在 100μg/mL 预孵育时将肿瘤坏死因子-α（TNF-α）水平降低至分别为 19.8、3.5、1.2 和 1.7ng/mL。这些馏分在 100μg/mL 时还明显抑制了 LPS 刺激细胞中白细胞介素（IL）-6 和 IL-1β 的分泌以及核因子-κB 的 DNA 结合活性。腹腔内注射 LPS（30mg/kg BW）的小鼠在注射后 48 小时的存活率为 20%，而馏分的口服给药将存活率提高到 80%。GC-MS 分析表明，馏分中存在苯甲酸和肉桂酸以及脂肪酸的衍生物。
大麦的地上部分可用作功能性食品，以预防急性炎症反应。
