Department of Applied Biochemistry, College of Biomedical and Health Science, Research Institute of Inflammatory Diseases, Konkuk University, Chungju 380-701, Korea.
Molecules. 2013 Jun 7;18(6):6663-78. doi: 10.3390/molecules18066663.
In this study, the acetylcholinesterase inhibition and in vitro and in vivo antioxidant activities of Ganoderma lucidum grown on germinated brown rice (GLBR) were evaluated. In antioxidant assays in vitro, GLBR was found to have strong metal chelating activity, DPPH, ABTS, hydroxyl and superoxide radical scavenging activity. Cell-based antioxidant methods were used, including lipid peroxidation on brain homogenate and AAPH-induced erythrocyte haemolysis. In antioxidant assays in vivo, mice were administered with GLBR and this significantly enhanced the activities of antioxidant enzymes in the mice sera, livers and brains. The amount of total phenolic and flavonoid compounds were 43.14 mg GAE/g and 13.36 mg CE/g dry mass, respectively. GLBR also exhibited acetylcholinesterase inhibitory activity. In addition, HPLC analyses of GLBR extract revealed the presence of different phenolic compounds. These findings demonstrate the remarkable potential of GLBR extract as valuable source of antioxidants which exhibit interesting acetylcholinesterase inhibitory activity.
在这项研究中，评估了在发芽糙米上生长的灵芝（GLBR）的乙酰胆碱酯酶抑制作用以及体外和体内抗氧化活性。在体外抗氧化测定中，发现 GLBR 具有很强的金属螯合活性、DPPH、ABTS、羟基和超氧自由基清除活性。使用基于细胞的抗氧化方法，包括脑匀浆中的脂质过氧化和 AAPH 诱导的红细胞溶血。在体内抗氧化测定中，给小鼠施用 GLBR，这显著增强了小鼠血清、肝脏和大脑中抗氧化酶的活性。总酚和类黄酮化合物的含量分别为 43.14 毫克 GAE/g 和 13.36 毫克 CE/g 干重。GLBR 还表现出乙酰胆碱酯酶抑制活性。此外，GLBR 提取物的 HPLC 分析表明存在不同的酚类化合物。这些发现表明 GLBR 提取物作为具有有趣乙酰胆碱酯酶抑制活性的抗氧化剂的有价值来源具有显著潜力。
