1Department of Obstetrics & Gynaecology, The University of Melbourne, Parkville, Victoria, Australia.
Reprod Sci. 2014 Jan;21(1):122-30. doi: 10.1177/1933719113492204. Epub 2013 Jun 7.
This work assessed the temporal coexpression of interleukin 1 (IL-1) and its inhibitor, IL-1 receptor antagonist (IL-1ra), in the cervicovaginal fluid (CVF) beyond 24 weeks gestation including women in spontaneous term labor. Two cohorts of women were recruited at 24 to 35 weeks' gestation (n = 65) and in late pregnancy (>36 weeks' gestation; n = 88). The CVF was serially collected either every 4 weeks between 24 and 35 weeks' gestation (n = 123 samples) or weekly during late pregnancy (n = 240 samples). The IL-1 and IL-1ra were quantitated by enzyme-linked immunosorbent assay, and the effect of vaginal microflora and unprotected sexual intercourse were also investigated. The IL-1β and IL-1ra remain unaltered between 24 and 35 weeks' gestation. At late pregnancy, IL-1α and β concentrations peak at 4 to 14 days prior to labor onset, while IL-1ra decreases with approaching spontaneous term labor (P < .05, 2-way analysis of variance). The IL-1 and IL-1ra were significantly correlated (P < .001, Pearson r). A combined biomarker model of IL-1α, IL-1β, and IL-1ra can predict term labor with 86% sensitivity and 92% specificity. This study indicates a shifting inflammatory balance in the gestational tissues prior to labor onset.
这项工作评估了白细胞介素 1（IL-1）及其抑制剂白细胞介素 1 受体拮抗剂（IL-1ra）在妊娠 24 周后宫颈阴道分泌物（CVF）中的时间协同表达，包括自发性足月分娩的女性。在妊娠 24 至 35 周（n = 65）和妊娠晚期（>36 周；n = 88）招募了两组女性。CVF 每隔 4 周采集一次，在妊娠 24 至 35 周之间（n = 123 个样本），或在妊娠晚期每周采集一次（n = 240 个样本）。通过酶联免疫吸附试验定量检测 IL-1 和 IL-1ra，并研究阴道微生物群和无保护性交的影响。IL-1β和 IL-1ra 在妊娠 24 至 35 周之间没有变化。在妊娠晚期，IL-1α和β浓度在分娩开始前 4 至 14 天达到峰值，而 IL-1ra 在接近自发性足月分娩时下降（P <.05，双向方差分析）。IL-1 和 IL-1ra 呈显著相关（P <.001，皮尔逊 r）。IL-1α、IL-1β 和 IL-1ra 的联合生物标志物模型可以预测足月分娩，其敏感性为 86%，特异性为 92%。本研究表明，在分娩开始前，妊娠组织中的炎症平衡发生了变化。
