噬菌体 PBECO4 感染大肠杆菌 O157:H7 的基因组分析。
Genomic analysis of bacteriophage PBECO4 infecting Escherichia coli O157:H7.
机构信息
Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yongin, Korea.
出版信息
Arch Virol. 2013 Nov;158(11):2399-403. doi: 10.1007/s00705-013-1718-3. Epub 2013 May 17.
Escherichia coli O157:H7 is a human pathogen. We isolated a novel bacteriophage infecting this bacterium from a sewage water treatment facility. Phage PBECO4 belongs to the family Myoviridae, having an isometric head and a contractile tail. It has a linear double-stranded DNA genome of 348,113 base pairs in length with a GC content of 34.09 %. Whole-genome sequencing revealed that PBECO4 is distantly related to enterobacteria phage vB_KleM_RaK2, with 10 % similarity, and Cronobacter phage vB_CsaM_GAP32 with 6 % similarity. Five hundred fifty-one putative open reading frames (ORFs) and six tRNA genes were found. Eight ORFs are related to genes encoding structural proteins, nine to DNA packaging, two to DNA lysis activity, and 42 to replication and regulation. Four hundred ninety ORFs have not been functionally annotated.
大肠杆菌 O157:H7 是人病原体。我们从污水处理设施中分离出一种感染该细菌的新型噬菌体。噬菌体 PBECO4 属于肌尾噬菌体科，具有等轴头和可收缩的尾巴。它具有 348113 个碱基对的线性双链 DNA 基因组，GC 含量为 34.09%。全基因组测序表明，PBECO4 与肠杆菌噬菌体 vB_KleM_RaK2 的相似度为 10%，与 Cronobacter 噬菌体 vB_CsaM_GAP32 的相似度为 6%。发现了 551 个推定的开放阅读框（ORFs）和 6 个 tRNA 基因。8 个 ORF 与编码结构蛋白的基因有关，9 个与 DNA 包装有关，2 个与 DNA 溶解释放活性有关，42 个与复制和调控有关。490 个 ORF 尚未具有功能注释。
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