Pharmaceutics Research Center, Kerman University of Medical Sciences, PO Box 76175-493, Kerman, Iran.
Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
Microbiology (Reading). 2013 Aug;159(Pt 8):1629-1638. doi: 10.1099/mic.0.063776-0. Epub 2013 May 15.
Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.
噬菌体被认为是控制传染病的有前途的生物制剂。对其基因组进行测序可以确定其是否存在抗生素耐药性、毒素或毒力基因。抗 O157:H7 噬菌体 PhaxI 是从伊朗的一个污水样本中分离出来的。透射电子显微镜的形态研究表明，它具有一个 85-86nm 的二十面体衣壳和一个 115×15nm 的收缩尾。PhaxI 含有由 156628nt 组成的 dsDNA，G+C 含量为 44.5mol%，编码 209 个推定蛋白。在噬菌体颗粒的 MS 分析中，鉴定出 92 种结构蛋白。PhaxI 在 LB 培养基和牛奶中裂解大肠杆菌 O157:H7，噬菌斑出现时间为 20 分钟，潜伏期为 40 分钟，每个细胞的爆发量约为 420 个。PhaxI 是肌尾噬菌体科“Viunalikevirus”属的成员，特异性针对大肠杆菌 O157:H7。
