The Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, USA ; Division of Orthopaedic Surgery, Toronto Western Hospital, University Health Network, Toronto, ON, Canada.
J Tissue Eng. 2013 Jun 9;4:2041731413492741. doi: 10.1177/2041731413492741. Print 2013.
To understand scar and adhesion formation during postsurgical period of intrasynovial tendon graft healing, a murine model of flexor digitorum longus tendon graft repair was developed, by utilizing flexor digitorum longus tendon allograft from donor Rosa26/+ mouse, and the healing process at days 3, 7, 14, 21, 28, and 35 post surgery of host wild-type mouse was followed. Using X-gal staining, β-galactosidase positive cells of allograft origin were detectable in tissue sections of grafted tendon post surgery. Graft healing was assessed for the cellular density, scar and adhesion formation, and their interaction with surrounding tissue. From histological analysis, it was evident that the healing of intrasynovial flexor digitorum longus tendon graft takes place in an interactive environment of donor graft, host tendon, and host surrounding tissue. A total of 32 genes, analyzed by RNA analysis, expressed during healing process. Particularly, Alk1, Postn, Tnc, Tppp3, and Mkx will be further investigated for therapeutical value in reducing scars and adhesions.
为了理解在滑液内肌腱移植物愈合后的术后阶段中瘢痕和粘连的形成，我们建立了一个利用来自供体 Rosa26/+ 小鼠的同种异体伸肌腱移植物的屈肌腱 longus 肌腱移植物修复的鼠模型，并在术后第 3、7、14、21、28 和 35 天对宿主野生型小鼠的愈合过程进行了跟踪。使用 X-gal 染色，在移植物肌腱的组织切片中可检测到供体移植物来源的β-半乳糖苷酶阳性细胞。对移植物的细胞密度、瘢痕和粘连形成及其与周围组织的相互作用进行了评估。从组织学分析中可以明显看出，滑液内屈肌腱 longus 肌腱移植物的愈合是在供体移植物、宿主肌腱和宿主周围组织的相互作用环境中发生的。通过 RNA 分析共分析了 32 个在愈合过程中表达的基因。特别是，Alk1、Postn、Tnc、Tppp3 和 Mkx 将进一步研究其在减少瘢痕和粘连方面的治疗价值。
