Vyas Narendra, Argal Ameeta
Institute of Pharmaceutical Science & Research Center, Bhagwant University, Ajmer, Rajasthan 305004, India ; Rajeev Gandhi College of Pharmacy, Village Salaiya, Via Danish Kunj, Kolar Road, Bhopal, Madhya Pradesh 462042, India.
ISRN Pharmacol. 2013 May 15;2013:951795. doi: 10.1155/2013/951795. Print 2013.
The present study was done to evaluate the antiurolithiatic activity of ethanolic extract of roots (ELC 200 mg/kg) and oleanolic acid (OA 60 mg/kg, O.A. 80 mg/kg, O.A. 100 mg/kg) isolated from roots of Lantana camara in albino wistar male rats using zinc disc implantation induced urolithiatic model. The group in which only zinc disc was implanted without any treatment showed increase in calcium output (23 ± 2.7 mg/dL). Cystone receiving animals showed significant protection from such change (P < 0.01). Treatment with OA and ELC significantly reduced the calcium output at a dose of OA 60 mg/kg (P < 0.01), OA 80 mg/kg (P < 0.01), ELC 200 mg/kg (P < 0.01), and OA 100 mg/kg (P < 0.001), as compared with zinc disc implanted group. The average weight of zinc discs along with the deposited crystals in the only disc implanted group was found to be 111 ± 8.6 mg. Group that received Cystone 500 mg/kg showed significant reduction in the depositions (P < 0.001). Similarly, the rats which received OA and ELC showed reduced formation of depositions around the zinc disc (P < 0.001). The X-ray images of rats also showed significant effect of OA and ELC on urolitiasis. Thus, OA and ELC showed promising antiurolithiatic activity in dose dependant manner.
本研究旨在使用锌盘植入诱导的尿石症模型，评估马缨丹根乙醇提取物（ELC 200毫克/千克）和从马缨丹根中分离出的齐墩果酸（OA 60毫克/千克、OA 80毫克/千克、OA 100毫克/千克）对白化Wistar雄性大鼠的抗尿石活性。仅植入锌盘而未进行任何处理的组钙排出量增加（23±2.7毫克/分升）。接受胱氨酸的动物对这种变化有显著的保护作用（P<0.01）。与仅植入锌盘的组相比，用OA和ELC治疗在OA 60毫克/千克（P<0.01）、OA 80毫克/千克（P<0.01）、ELC 200毫克/千克（P<0.01）和OA 100毫克/千克（P<0.001）剂量下显著降低了钙排出量。在仅植入锌盘的组中，发现锌盘连同沉积晶体的平均重量为111±8.6毫克。接受500毫克/千克胱氨酸的组沉积量显著减少（P<0.001）。同样，接受OA和ELC的大鼠在锌盘周围的沉积物形成减少（P<0.001）。大鼠的X射线图像也显示OA和ELC对尿石症有显著影响。因此，OA和ELC以剂量依赖方式显示出有前景的抗尿石活性。
