Mobergslien Anne, Sioud Mouldy
Institute for Cancer Research, Department of Immunology, Oslo University Hospital Radiumhospitalet, Oslo, Norway.
J Innate Immun. 2014;6(1):105-10. doi: 10.1159/000351460. Epub 2013 Jun 14.
Circulating exosome-containing small RNAs have been demonstrated in vitro to be taken up by recipient cells and to alter gene expression through RNA interference. Here, we show that exosomes purified from various cancer cell lines as well as gel-purified exosomal and cellular miRNAs can induce pro-inflammatory cytokine expression in human peripheral blood mononuclear cells. Thus, circulating miRNAs may trigger innate immunity via pathogen recognition receptors, a new miRNA-activated pathway that merits some consideration.
体外实验已证实，循环中含外泌体的小RNA可被受体细胞摄取，并通过RNA干扰改变基因表达。在此，我们发现，从各种癌细胞系中纯化得到的外泌体以及凝胶纯化的外泌体和细胞miRNA，均可诱导人外周血单核细胞表达促炎细胞因子。因此，循环miRNA可能通过病原体识别受体触发先天免疫，这是一条值得关注的新的miRNA激活途径。
