Prestes Letícia, Souza Beatriz M, Comar Lívia P, Salomão Priscila A, Rios Daniela, Magalhães Ana Carolina
Department of Biological Sciences, Bauru School of Dentistry/University of São Paulo, Al. Octávio Pinheiro Brisolla, 9-75, 17012-901 Bauru, SP, Brazil.
J Dent. 2013 Aug;41(8):747-51. doi: 10.1016/j.jdent.2013.06.006. Epub 2013 Jun 19.
Stimulation of salivary flow is considered a preventive strategy for dental erosion. Alternatively, products containing calcium phosphate, such as a complex of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), have also been tested against dental erosion. Therefore, this in situ study analyzed the effect of chewing gum containing CPP-ACP on the mineral precipitation of initial bovine enamel erosion lesions.
Twelve healthy adult subjects wore palatal appliances with two eroded bovine enamel samples. The erosion lesions were produced by immersion in 0.1% citric acid (pH 2.5) for 7 min. During three experimental crossover in situ phases (1 day each), the subjects chewed a type of gum, 3 times for 30 min, in each phase: with CPP-ACP (trident total), without CPP-ACP (trident), and no chewing gum (control). The Knoop surface microhardness was measured at baseline, after erosion in vitro and the mineral precipitation in situ. The differences in the degree of mineral precipitation were analyzed using repeated measures (RM-) ANOVA and post hoc Tukey's test (p<0.05).
Significant differences were found among the remineralizing treatments (p<0.0001). Chewing gum (19% of microhardness recovery) improved the mineral precipitation compared to control (10%) and the addition of CPP-ACP into the gum promoted the best mineral precipitation effect (30%).
Under this protocol, CPP-ACP chewing gum improved the mineral precipitation of eroded enamel.
Since the prevalence of dental erosion is steadily increasing, CPP-ACP chewing gum might be an important strategy to reduce the progression of initial erosion lesions.
刺激唾液分泌被认为是预防牙釉质侵蚀的一种策略。另外，含磷酸钙的产品，如酪蛋白磷酸肽 - 无定形磷酸钙（CPP - ACP）复合物，也已针对牙釉质侵蚀进行了测试。因此，本原位研究分析了含CPP - ACP的口香糖对初始牛牙釉质侵蚀病变矿物质沉淀的影响。
12名健康成年受试者佩戴带有两个侵蚀牛牙釉质样本的腭部矫治器。通过将样本浸泡在0.1%柠檬酸（pH 2.5）中7分钟产生侵蚀病变。在三个实验性交叉原位阶段（每个阶段1天）中，受试者在每个阶段咀嚼一种口香糖，每次30分钟，共咀嚼3次：含CPP - ACP（佳洁士全优护理口香糖）、不含CPP - ACP（佳洁士口香糖）和不咀嚼口香糖（对照）。在基线、体外侵蚀后和原位矿物质沉淀后测量努氏表面显微硬度。使用重复测量方差分析（RM - ANOVA）和事后Tukey检验分析矿物质沉淀程度的差异（p<0.05）。
再矿化处理之间存在显著差异（p<0.0001）。与对照（10%）相比，咀嚼口香糖（显微硬度恢复19%）改善了矿物质沉淀，并且在口香糖中添加CPP - ACP促进了最佳的矿物质沉淀效果（30%）。
在此方案下，含CPP - ACP的口香糖改善了侵蚀牙釉质的矿物质沉淀。
由于牙釉质侵蚀的患病率在稳步上升，含CPP - ACP的口香糖可能是减少初始侵蚀病变进展的重要策略。
