Chae Young Kee, Kim Seol Hyun, Ellinger James J, Markley John L
Department of Chemistry and Institute for Chemical Biology, Sejong University, Seoul 143-747, Korea.
Bull Korean Chem Soc. 2012 Dec 20;33(12):4041-4046. doi: 10.5012/bkcs.2012.33.12.4041.
The recombinant expression of proteins has been the method of choice to meet the demands from proteomics and structural genomics studies. Despite its successful production of many heterologous proteins, failed to produce many other proteins in their native forms. This may be related to the fact that the stresses resulting from the overproduction interfere with cellular processes. To better understand the physiological change during the overproduction phase, we profiled the metabolites along the time course of the recombinant protein expression. We identified 32 metabolites collected from different time points in the protein production phase. The stress induced by protein production can be characterized by (A) the increased usage of aspartic acid, choline, glycerol, and -acetyllysine; and (B) the accumulation of adenosine, alanine, oxidized glutathione, glycine, -acetylputrescine, and uracil. We envision that this work can be used to create a strategy for the production of usable proteins in large quantities.
蛋白质的重组表达一直是满足蛋白质组学和结构基因组学研究需求的首选方法。尽管它成功生产了许多异源蛋白质，但未能以天然形式生产许多其他蛋白质。这可能与过量生产所产生的压力干扰细胞过程这一事实有关。为了更好地了解过量生产阶段的生理变化，我们在重组蛋白表达的时间进程中对代谢物进行了分析。我们鉴定了在蛋白质生产阶段从不同时间点收集的32种代谢物。蛋白质生产所诱导的压力可表现为：（A）天冬氨酸、胆碱、甘油和N-乙酰赖氨酸的使用增加；以及（B）腺苷、丙氨酸、氧化型谷胱甘肽、甘氨酸、N-乙酰腐胺和尿嘧啶的积累。我们设想这项工作可用于制定大量生产可用蛋白质的策略。
